Quantitative Electron Microscopy in the Diagnosis of Mycosis Fungoides: A Simple Analysis of Lymphocytic Nuclear Convolutions

Claire M. Payne, Raymond B. Nagle, Peter J. Lynch

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

• In the present study we compared the accuracy of three different ultrastructural methods used to confirm clinically proved cases of mycosis fungoides (MF) from skin biopsy specimens. Method 1 is a qualitative method and confirmed the diagnosis of MF in only 44% of the patients with MF. Method 2 involved image analysis of lymphocytic nuclei and confirmed the diagnosis of MF in only 67% of the patients with MF. The most sensitive ultrastructural method for confirming the diagnosis of MF was a simple scoring of the number of sharply angled nuclear invaginations in 100 lymphocytes (method 3). Control biopsy specimens had many more lymphocytes (19% to 55%) with no sharply angled nuclear invaginations compared with those from patients with MF (3% to 15%). The clinical diagnosis of MF was confirmed in 100% of our patients with MF using method 3.

Original languageEnglish (US)
Pages (from-to)63-75
Number of pages13
JournalArchives of Dermatology
Volume120
Issue number1
DOIs
StatePublished - Jan 1984

ASJC Scopus subject areas

  • Dermatology

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