Quantitative light microscopic autoradiography of [3H]hemicholinium-3 binding sites in the rat central nervous system: a novel biochemical marker for mapping the distribution of cholinergic nerve terminals

Thomas W. Vickroy, William R. Roeske, Donald R. Gehlert, James K. Wamsley, Henry I. Yamamura

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

The distribution of specific [3H]hemicholinium-3 ([3H]HC-3) binding sites throughout the rat forebrain was studied by means of quantitative light microscopic autoradiography. Tissue sections were labeled with 2.5 nM[3H]HC-3, apposed to tritium-sensitive film for 2 months and analyzed by computer-assisted densitometry. Regions of intense [3H]HC-3 labeling include the caudate-putamen, nucleus accumbens, olfactory tubercle, amygdala, habenula and the granule cell layer of the dentate gyrus. Little or no specific binding was detected in the corpus callosum, a white matter region. This distribution of specific [3H]HC-3 binding sites is compatible with a selective labeling of central cholinergic nerve terminals.

Original languageEnglish (US)
Pages (from-to)368-373
Number of pages6
JournalBrain Research
Volume329
Issue number1-2
DOIs
StatePublished - Mar 11 1985
Externally publishedYes

Keywords

  • [H]hemicholinium-3
  • cholinergic nerve terminals
  • high-affinity choline uptake
  • light microscopic autoradiography

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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