Quantitative light microscopic autoradiography of [3H]hemicholinium-3 binding sites in the rat central nervous system: a novel biochemical marker for mapping the distribution of cholinergic nerve terminals

Thomas W. Vickroy, William R. Roeske, Donald R. Gehlert, James K. Wamsley, Henry I. Yamamura

Research output: Contribution to journalArticle

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The distribution of specific [3H]hemicholinium-3 ([3H]HC-3) binding sites throughout the rat forebrain was studied by means of quantitative light microscopic autoradiography. Tissue sections were labeled with 2.5 nM[3H]HC-3, apposed to tritium-sensitive film for 2 months and analyzed by computer-assisted densitometry. Regions of intense [3H]HC-3 labeling include the caudate-putamen, nucleus accumbens, olfactory tubercle, amygdala, habenula and the granule cell layer of the dentate gyrus. Little or no specific binding was detected in the corpus callosum, a white matter region. This distribution of specific [3H]HC-3 binding sites is compatible with a selective labeling of central cholinergic nerve terminals.

Original languageEnglish (US)
Pages (from-to)368-373
Number of pages6
JournalBrain Research
Issue number1-2
Publication statusPublished - Mar 11 1985
Externally publishedYes



  • cholinergic nerve terminals
  • high-affinity choline uptake
  • light microscopic autoradiography
  • [H]hemicholinium-3

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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