Quantitative specific binding of breast cancer cells in an antibody-functionalized microchamber array

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The system performance of an antibody-functionalized microchamber array, developed for selectively binding target cancer cells, is quantitatively characterized. The devices are designed to allow accurate counting of loaded and captured cells from heterogeneous suspensions. Specific interaction between cancer cell receptors and immobilized surface ligands has been demonstrated under static conditions. Performance measures such as specificity, sensitivity and accuracy are uniquely defined and experimentally evaluated. Several cell mixtures have been tested, and the quantitative system performance criteria are reported as a function of cell concentrations in the tested suspensions. These criteria provide a critical quantitative basis for a comparison among different systems aimed at selectively isolating target bio-species from complex mixtures.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages939-942
Number of pages4
DOIs
StatePublished - 2010
Event23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China
Duration: Jan 24 2010Jan 28 2010

Other

Other23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
CountryChina
CityHong Kong
Period1/24/101/28/10

Fingerprint

antibodies
Antibodies
breast
Suspensions
cancer
Cells
cells
Complex Mixtures
Ligands
counting
ligands
sensitivity
interactions

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Zheng, X. J., Cheung, L. S. L., Wang, L., Schroeder, J., Heimark, R. L., Baygents, J. C., ... Zohar, Y. (2010). Quantitative specific binding of breast cancer cells in an antibody-functionalized microchamber array. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 939-942). [5442362] https://doi.org/10.1109/MEMSYS.2010.5442362

Quantitative specific binding of breast cancer cells in an antibody-functionalized microchamber array. / Zheng, X. J.; Cheung, L. S L; Wang, L.; Schroeder, Joyce; Heimark, Ronald L; Baygents, James C; Guzman, Roberto Z; Zohar, Yitshak.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2010. p. 939-942 5442362.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zheng, XJ, Cheung, LSL, Wang, L, Schroeder, J, Heimark, RL, Baygents, JC, Guzman, RZ & Zohar, Y 2010, Quantitative specific binding of breast cancer cells in an antibody-functionalized microchamber array. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 5442362, pp. 939-942, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 1/24/10. https://doi.org/10.1109/MEMSYS.2010.5442362
Zheng XJ, Cheung LSL, Wang L, Schroeder J, Heimark RL, Baygents JC et al. Quantitative specific binding of breast cancer cells in an antibody-functionalized microchamber array. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2010. p. 939-942. 5442362 https://doi.org/10.1109/MEMSYS.2010.5442362
Zheng, X. J. ; Cheung, L. S L ; Wang, L. ; Schroeder, Joyce ; Heimark, Ronald L ; Baygents, James C ; Guzman, Roberto Z ; Zohar, Yitshak. / Quantitative specific binding of breast cancer cells in an antibody-functionalized microchamber array. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2010. pp. 939-942
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