Quantitative, surface heated, droplet polymerase chain reaction for detecting pathogens

Scott V. Angus, Soohee Cho, Dustin K. Harshman, Jeong-Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel quantitative polymerase chain reaction (qPCR) device was developed based on wire-guided droplet manipulation (WDM) method and subsequent real-time quantification utilizing a smartphone or optical fiber/miniature spectrophotometer. The device was initially tested to amplify GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene and further tested to identify Escherichia coli. The lower limit of detection was ∼2 copies per sample. The device is portable with real-time quantification and provides the assay results quickly (30-cycle amplification for 7-20 min) and accurately. The system is also shock and vibration resistant.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1452-1454
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Contact angle
  • Escherichia coli
  • Real-time polymerase chain reaction

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Angus, S. V., Cho, S., Harshman, D. K., & Yoon, J-Y. (2014). Quantitative, surface heated, droplet polymerase chain reaction for detecting pathogens. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1452-1454). Chemical and Biological Microsystems Society.