Quasi-elastic Neutron Scattering Reveals Ligand-Induced Protein Dynamics of a G-Protein-Coupled Receptor

Utsab R. Shrestha, Suchithranga M D C Perera, Debsindhu Bhowmik, Udeep Chawla, Eugene Mamontov, Michael F Brown, Xiang Qiang Chu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Light activation of the visual G-protein-coupled receptor (GPCR) rhodopsin leads to significant structural fluctuations of the protein embedded within the membrane yielding the activation of cognate G-protein (transducin), which initiates biological signaling. Here, we report a quasi-elastic neutron scattering study of the activation of rhodopsin as a GPCR prototype. Our results reveal a broadly distributed relaxation of hydrogen atom dynamics of rhodopsin on a picosecond-nanosecond time scale, crucial for protein function, as only observed for globular proteins previously. Interestingly, the results suggest significant differences in the intrinsic protein dynamics of the dark-state rhodopsin versus the ligand-free apoprotein, opsin. These differences can be attributed to the influence of the covalently bound retinal ligand. Furthermore, an idea of the generic free-energy landscape is used to explain the GPCR dynamics of ligand-binding and ligand-free protein conformations, which can be further applied to other GPCR systems.

Original languageEnglish (US)
Pages (from-to)4130-4136
Number of pages7
JournalJournal of Physical Chemistry Letters
Volume7
Issue number20
DOIs
StatePublished - Oct 20 2016

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Elastic scattering
G-Protein-Coupled Receptors
Neutron scattering
Rhodopsin
elastic scattering
neutron scattering
Ligands
proteins
Proteins
ligands
Chemical activation
Transducin
Opsins
Apoproteins
activation
GTP-Binding Proteins
Free energy
Conformations
Hydrogen
Membranes

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Quasi-elastic Neutron Scattering Reveals Ligand-Induced Protein Dynamics of a G-Protein-Coupled Receptor. / Shrestha, Utsab R.; Perera, Suchithranga M D C; Bhowmik, Debsindhu; Chawla, Udeep; Mamontov, Eugene; Brown, Michael F; Chu, Xiang Qiang.

In: Journal of Physical Chemistry Letters, Vol. 7, No. 20, 20.10.2016, p. 4130-4136.

Research output: Contribution to journalArticle

Shrestha, Utsab R. ; Perera, Suchithranga M D C ; Bhowmik, Debsindhu ; Chawla, Udeep ; Mamontov, Eugene ; Brown, Michael F ; Chu, Xiang Qiang. / Quasi-elastic Neutron Scattering Reveals Ligand-Induced Protein Dynamics of a G-Protein-Coupled Receptor. In: Journal of Physical Chemistry Letters. 2016 ; Vol. 7, No. 20. pp. 4130-4136.
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AU - Chawla, Udeep

AU - Mamontov, Eugene

AU - Brown, Michael F

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