@inbook{e172c1f7619148a5b6a9641307d4caba,
title = "QueE: A Radical SAM Enzyme Involved in the Biosynthesis of 7-Deazapurine Containing Natural Products",
abstract = "7-Carboxy-7-deazaguanine (CDG) is a common intermediate in the biosynthesis of 7-deazapurine-containing natural products. The biosynthesis of CDG from GTP requires three enzymes: GTP cyclohydrolase I, 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) synthase, and CDG synthase (QueE). QueE is a member of the radical S-adenosyl-L-methionine (SAM) superfamily and catalyzes the SAM-dependent radical-mediated ring contraction of CPH4 to generate CDG. This chapter focuses on methods to reconstitute the activity of QueE in vitro.",
keywords = "7-Deazapurine, CDG synthase, Radical SAM, tRNA modification",
author = "Lewis, {Julia K.} and Bruender, {Nathan A.} and Vahe Bandarian",
note = "Funding Information: This work was supported by the National Institutes of Health (GM072623 and GM120638). Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2018",
doi = "10.1016/bs.mie.2018.05.001",
language = "English (US)",
isbn = "9780128127940",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "95--118",
editor = "Vahe Bandarian",
booktitle = "Methods in Enzymology",
}