QueE: A Radical SAM Enzyme Involved in the Biosynthesis of 7-Deazapurine Containing Natural Products

Julia K. Lewis; Nathan A. Bruender; Vahe Bandarian

doi:10.1016/bs.mie.2018.05.001

QueE: A Radical SAM Enzyme Involved in the Biosynthesis of 7-Deazapurine Containing Natural Products

Julia K. Lewis, Nathan A. Bruender, Vahe Bandarian

Research output: Contribution to journal › Article

1 Scopus citations

Abstract

7-Carboxy-7-deazaguanine (CDG) is a common intermediate in the biosynthesis of 7-deazapurine-containing natural products. The biosynthesis of CDG from GTP requires three enzymes: GTP cyclohydrolase I, 6-carboxy-5,6,7,8-tetrahydropterin (CPH₄) synthase, and CDG synthase (QueE). QueE is a member of the radical S-adenosyl-L-methionine (SAM) superfamily and catalyzes the SAM-dependent radical-mediated ring contraction of CPH₄ to generate CDG. This chapter focuses on methods to reconstitute the activity of QueE in vitro.

Original language	English (US)
Journal	Methods in Enzymology
DOIs	https://doi.org/10.1016/bs.mie.2018.05.001
State	Accepted/In press - Jan 1 2018
Externally published	Yes

Keywords

7-Deazapurine
CDG synthase
Radical SAM
tRNA modification

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/bs.mie.2018.05.001

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Lewis, J. K., Bruender, N. A., & Bandarian, V. (Accepted/In press). QueE: A Radical SAM Enzyme Involved in the Biosynthesis of 7-Deazapurine Containing Natural Products. Methods in Enzymology. https://doi.org/10.1016/bs.mie.2018.05.001

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abstract = "7-Carboxy-7-deazaguanine (CDG) is a common intermediate in the biosynthesis of 7-deazapurine-containing natural products. The biosynthesis of CDG from GTP requires three enzymes: GTP cyclohydrolase I, 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) synthase, and CDG synthase (QueE). QueE is a member of the radical S-adenosyl-L-methionine (SAM) superfamily and catalyzes the SAM-dependent radical-mediated ring contraction of CPH4 to generate CDG. This chapter focuses on methods to reconstitute the activity of QueE in vitro.",

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