Abstract
7-Carboxy-7-deazaguanine (CDG) is a common intermediate in the biosynthesis of 7-deazapurine-containing natural products. The biosynthesis of CDG from GTP requires three enzymes: GTP cyclohydrolase I, 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) synthase, and CDG synthase (QueE). QueE is a member of the radical S-adenosyl-L-methionine (SAM) superfamily and catalyzes the SAM-dependent radical-mediated ring contraction of CPH4 to generate CDG. This chapter focuses on methods to reconstitute the activity of QueE in vitro.
| Original language | English (US) |
|---|---|
| Journal | Methods in Enzymology |
| DOIs | |
| State | Accepted/In press - Jan 1 2018 |
| Externally published | Yes |
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Keywords
- 7-Deazapurine
- CDG synthase
- Radical SAM
- tRNA modification
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
QueE : A Radical SAM Enzyme Involved in the Biosynthesis of 7-Deazapurine Containing Natural Products. / Lewis, Julia K.; Bruender, Nathan A.; Bandarian, Vahe.
In: Methods in Enzymology, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - QueE
T2 - A Radical SAM Enzyme Involved in the Biosynthesis of 7-Deazapurine Containing Natural Products
AU - Lewis, Julia K.
AU - Bruender, Nathan A.
AU - Bandarian, Vahe
PY - 2018/1/1
Y1 - 2018/1/1
N2 - 7-Carboxy-7-deazaguanine (CDG) is a common intermediate in the biosynthesis of 7-deazapurine-containing natural products. The biosynthesis of CDG from GTP requires three enzymes: GTP cyclohydrolase I, 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) synthase, and CDG synthase (QueE). QueE is a member of the radical S-adenosyl-L-methionine (SAM) superfamily and catalyzes the SAM-dependent radical-mediated ring contraction of CPH4 to generate CDG. This chapter focuses on methods to reconstitute the activity of QueE in vitro.
AB - 7-Carboxy-7-deazaguanine (CDG) is a common intermediate in the biosynthesis of 7-deazapurine-containing natural products. The biosynthesis of CDG from GTP requires three enzymes: GTP cyclohydrolase I, 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) synthase, and CDG synthase (QueE). QueE is a member of the radical S-adenosyl-L-methionine (SAM) superfamily and catalyzes the SAM-dependent radical-mediated ring contraction of CPH4 to generate CDG. This chapter focuses on methods to reconstitute the activity of QueE in vitro.
KW - 7-Deazapurine
KW - CDG synthase
KW - Radical SAM
KW - tRNA modification
UR - http://www.scopus.com/inward/record.url?scp=85049727792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049727792&partnerID=8YFLogxK
U2 - 10.1016/bs.mie.2018.05.001
DO - 10.1016/bs.mie.2018.05.001
M3 - Article
C2 - 30097106
AN - SCOPUS:85049727792
JO - Methods
JF - Methods
SN - 0076-6879
ER -