QueE: A Radical SAM Enzyme Involved in the Biosynthesis of 7-Deazapurine Containing Natural Products

Julia K. Lewis, Nathan A. Bruender, Vahe Bandarian

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

7-Carboxy-7-deazaguanine (CDG) is a common intermediate in the biosynthesis of 7-deazapurine-containing natural products. The biosynthesis of CDG from GTP requires three enzymes: GTP cyclohydrolase I, 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) synthase, and CDG synthase (QueE). QueE is a member of the radical S-adenosyl-L-methionine (SAM) superfamily and catalyzes the SAM-dependent radical-mediated ring contraction of CPH4 to generate CDG. This chapter focuses on methods to reconstitute the activity of QueE in vitro.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsVahe Bandarian
PublisherAcademic Press Inc.
Pages95-118
Number of pages24
ISBN (Print)9780128127940
DOIs
StatePublished - 2018

Publication series

NameMethods in Enzymology
Volume606
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • 7-Deazapurine
  • CDG synthase
  • Radical SAM
  • tRNA modification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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