QueE: A Radical SAM Enzyme Involved in the Biosynthesis of 7-Deazapurine Containing Natural Products

Julia K. Lewis, Nathan A. Bruender, Vahe Bandarian

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

7-Carboxy-7-deazaguanine (CDG) is a common intermediate in the biosynthesis of 7-deazapurine-containing natural products. The biosynthesis of CDG from GTP requires three enzymes: GTP cyclohydrolase I, 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) synthase, and CDG synthase (QueE). QueE is a member of the radical S-adenosyl-L-methionine (SAM) superfamily and catalyzes the SAM-dependent radical-mediated ring contraction of CPH4 to generate CDG. This chapter focuses on methods to reconstitute the activity of QueE in vitro.

Original languageEnglish (US)
JournalMethods in Enzymology
DOIs
StateAccepted/In press - Jan 1 2018
Externally publishedYes

Keywords

  • 7-Deazapurine
  • CDG synthase
  • Radical SAM
  • tRNA modification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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