RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast

Richard Gardner, Charles W. Putnam, Ted Weinert

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Eukaryotic checkpoint genes regulate multiple cellular responses to DNA damage. In this report, we examine the roles of budding yeast genes involved in G2/M arrest and tolerance to UV exposure. A current model posits three gene classes: those encoding proteins acting on damaged DNA (e.g. RAD9 and RAD24), those transducing a signal (MEC1, RAD53 and DUN1) or those participating more directly in arrest (PDS1). Here, we define important features of the pathways subserved by those genes. MEC1, which we find is required for both establishment and maintenance of G2/M arrest, mediates this arrest through two parallel pathways. One pathway requires RAD53 and DUN1 (the 'RAD53 pathway'); the other pathway requires PDS1. Each pathway independently contributes ~ 50% to G2/M arrest, effects demonstrable after cdc13-induced damage or a double-stranded break inflicted by the HO endonuclease. Similarly, both pathways contribute independently to tolerance of UV irradiation. How the parallel pathways might interact ultimately to achieve arrest is not yet understood, but we do provide evidence that neither the RAD53 nor the PDS1 pathway appears to maintain arrest by inhibiting adaptation. Instead, we think it likely that both pathways contribute to establishing and maintaining arrest.

Original languageEnglish (US)
Pages (from-to)3173-3185
Number of pages13
JournalEMBO Journal
Volume18
Issue number11
DOIs
StatePublished - Jun 1 1999

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Saccharomycetales
Yeast
Genes
Endonucleases
DNA
DNA Damage
Maintenance
Irradiation
Proteins

Keywords

  • Checkpoint
  • DNA damage
  • Mitotic arreste
  • Pds1
  • Rad53

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast. / Gardner, Richard; Putnam, Charles W.; Weinert, Ted.

In: EMBO Journal, Vol. 18, No. 11, 01.06.1999, p. 3173-3185.

Research output: Contribution to journalArticle

Gardner, Richard ; Putnam, Charles W. ; Weinert, Ted. / RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast. In: EMBO Journal. 1999 ; Vol. 18, No. 11. pp. 3173-3185.
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