Radiation hybrid mapping: A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes

David R. Cox, Margit Burmeister, E. Roydon Price, Suwon Kim, Richard M. Myers

Research output: Contribution to journalArticle

569 Scopus citations

Abstract

Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes.

Original languageEnglish (US)
Pages (from-to)245-250
Number of pages6
JournalScience
Volume250
Issue number4980
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Radiation hybrid mapping: A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes'. Together they form a unique fingerprint.

  • Cite this