Radiation hybrid mapping: A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes

David R. Cox, Margit Burmeister, E. Roydon Price, Suwon Kim, Richard M. Myers

Research output: Contribution to journalArticle

564 Citations (Scopus)

Abstract

Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes.

Original languageEnglish (US)
Pages (from-to)245-250
Number of pages6
JournalScience
Volume250
Issue number4980
StatePublished - 1990
Externally publishedYes

Fingerprint

Mammalian Chromosomes
Radiation Hybrid Mapping
Genetic Techniques
Chromosome Breakage
Chromosomes, Human, Pair 21
DNA Probes
Human Chromosomes
Genetic Markers
Chromosomes
Gels
X-Rays

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Radiation hybrid mapping : A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes. / Cox, David R.; Burmeister, Margit; Price, E. Roydon; Kim, Suwon; Myers, Richard M.

In: Science, Vol. 250, No. 4980, 1990, p. 245-250.

Research output: Contribution to journalArticle

Cox, DR, Burmeister, M, Price, ER, Kim, S & Myers, RM 1990, 'Radiation hybrid mapping: A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes', Science, vol. 250, no. 4980, pp. 245-250.
Cox, David R. ; Burmeister, Margit ; Price, E. Roydon ; Kim, Suwon ; Myers, Richard M. / Radiation hybrid mapping : A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes. In: Science. 1990 ; Vol. 250, No. 4980. pp. 245-250.
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