Radiation-induced changes in nucleoid halo diameters of aerobic and hypoxic SF-126 human brain tumor cells

J. Wang, H. S. Basu, L. Hu, Burt G.F. Feuerstein, P. M. Nederlof, D. F. Deen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Nucleoid halo diameters were measured to assay changes in DNA supercoiling in human brain tumor cell line SF-126 after irradiation under aerobic or hypoxic conditions. In unirradiated aerobic cells, a typical propidium iodide titration curve showed that with increasing concentrations of propidium iodide, the halo diameter increased and then decreased with the unwinding and subsequent rewinding of DNA supercoils. In irradiated cells, the rewinding of DNA supercoils was inhibited, resulting in an increased halo diameter, in a radiation dose-dependent manner. To produce equal increases in halo diameter required about a threefold higher radiation dose in hypoxic cells than in aerobic cells. Quantitatively similar differences in the radiation sensitivities of hypoxic and aerobic cells were demonstrated by a colony-forming efficiency assay. These findings suggest that the nucleoid halo assay may be used as a rapid measure of the inherent radiation sensitivity of human tumors.

Original languageEnglish (US)
Pages (from-to)107-111
Number of pages5
JournalCytometry
Volume19
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Brain Neoplasms
Radiation
Propidium
Radiation Tolerance
DNA
Tumor Cell Line
Neoplasms

Keywords

  • DNA supercoil
  • Human brain tumor cells
  • Hypoxia
  • Nucleoid halo
  • Propidium iodide-DNA complex
  • Radiation

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Radiation-induced changes in nucleoid halo diameters of aerobic and hypoxic SF-126 human brain tumor cells. / Wang, J.; Basu, H. S.; Hu, L.; Feuerstein, Burt G.F.; Nederlof, P. M.; Deen, D. F.

In: Cytometry, Vol. 19, No. 2, 1995, p. 107-111.

Research output: Contribution to journalArticle

Wang, J. ; Basu, H. S. ; Hu, L. ; Feuerstein, Burt G.F. ; Nederlof, P. M. ; Deen, D. F. / Radiation-induced changes in nucleoid halo diameters of aerobic and hypoxic SF-126 human brain tumor cells. In: Cytometry. 1995 ; Vol. 19, No. 2. pp. 107-111.
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