Radiation treatment decreases bone cancer pain through direct effect on tumor cells

Michael Goblirsch, Christine Lynch, Wendy Mathews, J. Carlos Manivel, Patrick W Mantyh, Denis R. Clohisy

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The most used treatment for bone cancer pain is radiation; however, the mechanism responsible for analgesia after irradiation is unknown. The mechanistic influence of a single, localized 10-, 20- or 30-Gy dose of radiation on painful behaviors, osteolysis, histopathology and osteoclast number was evaluated in mice with painful femoral sarcomas. Dramatic reductions in pain behaviors (P < 0.05) and osteolysis (P < 0.0001) were seen in mice irradiated with 20 and 30 Gy. Irradiation reduced the tumor area by more than 75% (P < 0.05) but did not affect osteoclast frequency per mm2 tumor. Treatment with 20 Gy prior to tumor injection had no effect on tumor growth or pain behaviors, suggesting that radiation reduces osteolysis and pain through direct tumor effects. To demonstrate that tumor elimination was responsible for reduction in osteolysis and pain, sarcoma cells containing the suicide gene cytosine deaminase (CD) were inoculated into femora. After onset of bone cancer pain, mice were treated with the prodrug 5-fluorocytosine (5-FC). 5-FC treatment significantly reduced both osteolysis (P < 0.0005) and bone cancer pain (P < 0.05). The findings in this study demonstrate that one mechanism through which radiation decreases bone cancer pain is by direct effects on tumor cells.

Original languageEnglish (US)
Pages (from-to)400-408
Number of pages9
JournalRadiation Research
Volume164
Issue number4 I
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Bone Neoplasms
pain
bones
Osteolysis
tumors
cancer
Radiation
neoplasms
radiation
Neoplasms
Flucytosine
Pain
mice
Osteoclasts
Sarcoma
Therapeutics
osteoclasts
sarcoma
Cytosine Deaminase
analgesia

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Goblirsch, M., Lynch, C., Mathews, W., Manivel, J. C., Mantyh, P. W., & Clohisy, D. R. (2005). Radiation treatment decreases bone cancer pain through direct effect on tumor cells. Radiation Research, 164(4 I), 400-408. https://doi.org/10.1667/RR3439.1

Radiation treatment decreases bone cancer pain through direct effect on tumor cells. / Goblirsch, Michael; Lynch, Christine; Mathews, Wendy; Manivel, J. Carlos; Mantyh, Patrick W; Clohisy, Denis R.

In: Radiation Research, Vol. 164, No. 4 I, 10.2005, p. 400-408.

Research output: Contribution to journalArticle

Goblirsch, M, Lynch, C, Mathews, W, Manivel, JC, Mantyh, PW & Clohisy, DR 2005, 'Radiation treatment decreases bone cancer pain through direct effect on tumor cells', Radiation Research, vol. 164, no. 4 I, pp. 400-408. https://doi.org/10.1667/RR3439.1
Goblirsch, Michael ; Lynch, Christine ; Mathews, Wendy ; Manivel, J. Carlos ; Mantyh, Patrick W ; Clohisy, Denis R. / Radiation treatment decreases bone cancer pain through direct effect on tumor cells. In: Radiation Research. 2005 ; Vol. 164, No. 4 I. pp. 400-408.
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