Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics

Rachel Meyer, Wen Yu Wong, Roberto Guzman, Randy M Burd, Kirsten Limesand

Research output: Contribution to journalArticle

Abstract

Hyposalivation and xerostomia create chronic oral complications that decrease the quality of life in head and neck cancer patients who are treated with radiotherapy. Experimental approaches to understanding mechanisms of salivary gland dysfunction and restoration have focused on in vivo models, which are handicapped by an inability to systematically screen therapeutic candidates and efficiencies in transfection capability to manipulate specific genes. The purpose of this salivary gland organotypic culture protocol is to evaluate maximal time of culture viability and characterize cellular changes following ex vivo radiation treatment. We utilized immunofluorescent staining and confocal microscopy to determine when specific cell populations and markers are present during a 30-day culture period. In addition, cellular markers previously reported in in vivo radiation models are evaluated in cultures that are irradiated ex vivo. Moving forward, this method is an attractive platform for rapid ex vivo assessment of murine and human salivary gland tissue responses to therapeutic agents that improve salivary function.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number147
DOIs
StatePublished - May 17 2019

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Parotid Gland
Salivary Glands
Xerostomia
Radiation
Confocal microscopy
Radiotherapy
Restoration
Genes
Cells
Tissue
Head and Neck Neoplasms
Confocal Microscopy
Transfection
Therapeutics
Quality of Life
Staining and Labeling
Population

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

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