Radioimmunoassay for phencyclidine (PCP) in serum

S. M. Owens, J. Woodworth, M. Mayersohn

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

This accurate, sensitive radioimmunoassay for determining phencyclidine concentrations in serum specimens involves the use of anti-phencyclidine sera, 0.1 mL of serum specimen, an iodinated tracer, and a solid-phase separation. Phencyclidine metabolites do not show significant cross reactivity, but several phencyclidine analogs do cross react. Within-run coefficients of variation for human and dog serum ranged from 2.5 to 13% for concentrations from 2.0 to 500 μg/L. Day-to-day coefficients of variation for human and dog serum ranged from 4.3 to 16.7% for concentrations ranging from 2.0 to 90.0 μg/L. The sensitivity of the radioimmunoassay is < 0.5 μg/L. Thirty serum specimens from two dogs given 1 mg of phencyclidine per kilogram body weight were analyzed by radioimmunoassay and a gas-chromatographic method. Nonparametric statistical comparison and linear regression showed that results from the two procedures correlate well (r2 = 0.952). Concentration-time data from the two dogs are presented to illustrate the utility of the radioimmunoassay for examining phencyclidine disposition.

Original languageEnglish (US)
Pages (from-to)1509-1513
Number of pages5
JournalClinical chemistry
Volume28
Issue number7
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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