Radioimmunoassay for phencyclidine (PCP) in serum

S. M. Owens, J. Woodworth, Michael Mayersohn

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This accurate, sensitive radioimmunoassay for determining phencyclidine concentrations in serum specimens involves the use of anti-phencyclidine sera, 0.1 mL of serum specimen, an iodinated tracer, and a solid-phase separation. Phencyclidine metabolites do not show significant cross reactivity, but several phencyclidine analogs do cross react. Within-run coefficients of variation for human and dog serum ranged from 2.5 to 13% for concentrations from 2.0 to 500 μg/L. Day-to-day coefficients of variation for human and dog serum ranged from 4.3 to 16.7% for concentrations ranging from 2.0 to 90.0 μg/L. The sensitivity of the radioimmunoassay is < 0.5 μg/L. Thirty serum specimens from two dogs given 1 mg of phencyclidine per kilogram body weight were analyzed by radioimmunoassay and a gas-chromatographic method. Nonparametric statistical comparison and linear regression showed that results from the two procedures correlate well (r2 = 0.952). Concentration-time data from the two dogs are presented to illustrate the utility of the radioimmunoassay for examining phencyclidine disposition.

Original languageEnglish (US)
Pages (from-to)1509-1513
Number of pages5
JournalClinical Chemistry
Volume28
Issue number7
StatePublished - 1982

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Phencyclidine
Radioimmunoassay
Serum
Dogs
Metabolites
Linear regression
Phase separation
Linear Models
Gases
Body Weight
Regression Analysis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Owens, S. M., Woodworth, J., & Mayersohn, M. (1982). Radioimmunoassay for phencyclidine (PCP) in serum. Clinical Chemistry, 28(7), 1509-1513.

Radioimmunoassay for phencyclidine (PCP) in serum. / Owens, S. M.; Woodworth, J.; Mayersohn, Michael.

In: Clinical Chemistry, Vol. 28, No. 7, 1982, p. 1509-1513.

Research output: Contribution to journalArticle

Owens, SM, Woodworth, J & Mayersohn, M 1982, 'Radioimmunoassay for phencyclidine (PCP) in serum', Clinical Chemistry, vol. 28, no. 7, pp. 1509-1513.
Owens, S. M. ; Woodworth, J. ; Mayersohn, Michael. / Radioimmunoassay for phencyclidine (PCP) in serum. In: Clinical Chemistry. 1982 ; Vol. 28, No. 7. pp. 1509-1513.
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