Radionuclide imaging of acute lung transplant rejection with annexin V

Francis G. Blankenberg, Robert C. Robbins, Jan H. Stoot, Patrick W. Vriens, Gerald J. Berry, Jonathan F. Tait, H. William Strauss

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Study objectives: Early detection and treatment of lung transplant rejection is critical for preservation of pulmonary graft function. Damage to pulmonary allografts is mediated by apoptotic cell death induced by the alloreactive T lymphocytes that infiltrate lung grafts. Previous studies demonstrate that acute cardiac allograft rejection can be visualized using radiolabeled annexin V. This study was done to determine whether this technique could visualize acute rejection in a rodent model of unilateral orthotopic lung transplantation. Design: Eighteen Sprague-Dawley ACI rats underwent removal of their left lung followed by orthotopic transplant of either an allogeneic (PVG, immunologically mismatched; N = 10) or a syngeneic (ACI, immunologically matched) pulmonary graft (N = 8). Animals were imaged 1 h after IV injection of 1 mCi (37.0 MBq) of 99mTc-annexin V 1 to 7 days after transplantation. Results: Lungs receiving the allograft demonstrated moderate to marked mononuclear infiltration of the perivascular, interstitial, and peribronchial tissues. No mononuclear infiltrates were noted in the native right lungs nor in the syngeneic transplants. Region of interest image analysis revealed significant (p < 0.0005) increases of transplant to normal lung activity ratios 3 to 7 days after allograft surgery. The increased annexin V uptake in these lungs was confirmed at biodistribution assay (allograft 151% greater than isograft activity, p < 0.005). Conclusions: Acute experimental lung transplant rejection can be noninvasively identified using 99mTc-annexin V. Radiolabeled annexin V may be a clinically useful noninvasive screening tool for acute rejection.

Original languageEnglish (US)
Pages (from-to)834-840
Number of pages7
JournalCHEST
Volume117
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Acute rejection
  • Apoptosis
  • Lymphocytes
  • Organ transplantation
  • Pulmonary
  • Radionuclide imaging

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine
  • Cardiology and Cardiovascular Medicine

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