Radioreceptor assay for 1α,25-dihydroxyvitamin D3

Peter F. Brumbaugh, David H. Haussler, Rubin Bressler, Mark R. Haussler

Research output: Contribution to journalArticlepeer-review

130 Scopus citations

Abstract

A comtipetitive protein binding assay with a sensitivity of 80 picograms has been developed for 1α,25-dihydroxyvitamin D3, the hormonal form of vitamin D3. 1α,25-Dihydroxyvitamin D3 displaced tritiated hormone from a cytosol-chromatin receptor preparation isolated from chick small intestine, providing a simple assay for the hormone. The concentration of 1α,25-dihydroxyvitamin D3 in human plasma, as determined by this assay, is approximately 6 nanograms per 100 milliliters; in patients with renal disease the concentration of this kidney-produced hormone is significantly lower.

Original languageEnglish (US)
Pages (from-to)1089-1091
Number of pages3
JournalScience
Volume183
Issue number4129
DOIs
StatePublished - Jan 1 1974

ASJC Scopus subject areas

  • General

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