Rapid analysis of apolar low molecular weight constituents in wood using high pressure liquid chromatography with evaporative light scattering detection

Frank W. Claassen, Cees Van De Haar, Teris A. Van Beek, José Dorado, María Jose Martínez-Iñigo, Reyes Sierra-Alvarez

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

A new high pressure liquid chromatographic method with evaporative light scattering detection was developed for the qualitative and quantitative analysis of apolar, low molecular weight constituents in wood. The wood extractives were obtained by means of a 6 h Soxhlet extraction with acetone. The extract was quantitatively analysed on an end-capped C18 column eluted with a gradient of acetonitrile:water:acetic acid. The crude sample was directly injected following dissolution in acetonitrile:methanol:dichloromethane (60:8:40) and filtration through a 0.45 μm membrane filter. No other time consuming fractionation or derivatisation steps were required prior to the analysis. The main apolar, low molecular weight constituents of pinewood are free fatty acids, resin acids, sterols and triglycerides. With this method, individual free fatty acids, sterols and triglycerides are well separated. Resin acids, with the exception of dehydroabietic acid, are not separated and co-elute. The method does not require any sample clean-up steps except filtration and it is compatible with other softwood and hardwood wood species. Copyright (C) 2000 John Wiley and Sons, Ltd.

Original languageEnglish (US)
Pages (from-to)251-256
Number of pages6
JournalPhytochemical Analysis
Volume11
Issue number4
DOIs
StatePublished - Jul 1 2000
Externally publishedYes

Keywords

  • Evaporative light scattering detection
  • Fatty acids
  • Fungi
  • High performance liquid chromatography
  • Pitch
  • Resin
  • Resin acids
  • Scots pine
  • Sterols
  • Triglycerides
  • Wood extractives

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

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