Rapid and efficient regeneration of plants from explants of Arabidopsis thaliana

Kenneth A. Feldmann, M. David Marks

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

A procedure is given for the regeneration in vitro of a previously difficult to regenerate plant, Arabidopsis thaliana (L.) Heynh. Leaf explants of Arabidopsis that are given a short exposure to a callus-inducing medium prior to incubation on a shoot-inducing medium exhibit high survivability and rapidly produce shoots. Some media combinations allow shoots to form on 100% of the explants and the majority of these explants form multiple shoots. The shoots are normal in appearance and most of them can be induced to form functional roots and retain fertility. This regeneration scheme should be of use in the development of an Arabidopsis transformation procedure and may also be applicable to other recalcitrant plant species.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalPlant Science
Volume47
Issue number1
DOIs
StatePublished - 1986
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • competence
  • plant regeneration
  • plant tissue culture
  • suspension culture

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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