Rapid and sensitive detection of malaria antigen in human blood with lab-on-a-chip

C. Christopher Stemple, Hyuck Jin Kwon, Jeong-Yeol Yoon

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A novel lab-on-a-chip detection device, based on the properties of immunoagglutination, was modified to detect malaria in the human blood through histidine-rich protein 2, an antigen expressed only by Plasmodium falciparum. Utilizing Mie scattering detection, which is angle- and size-dependent, the extent of immunoagglutination could be accurately measured while the optical disturbance from the human blood was minimized. The presence of human serum albumin in the blood is believed to further stabilize the antibody-conjugated submicron beads and/or break off the larger agglutinated beads. The lowest detection limit was 1 pg/mL in 10% of whole blood (equivalent to 10 pg/mL in undiluted whole blood), a few orders of magnitude lower than other assays. The final device is compact, with a fast assay time of approximately 8 min.

Original languageEnglish (US)
Article number6221942
Pages (from-to)2735-2736
Number of pages2
JournalIEEE Sensors Journal
Volume12
Issue number9
DOIs
StatePublished - 2012

Fingerprint

Lab-on-a-chip
antigens
Antigens
blood
Blood
chips
beads
Assays
histidine
Mie scattering
antibodies
albumins
Antibodies
serums
disturbances
Scattering
proteins
Proteins

Keywords

  • Latex immunoagglutination
  • microfluidics
  • Mie scatter
  • plasmodium falciparum

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Instrumentation

Cite this

Rapid and sensitive detection of malaria antigen in human blood with lab-on-a-chip. / Stemple, C. Christopher; Kwon, Hyuck Jin; Yoon, Jeong-Yeol.

In: IEEE Sensors Journal, Vol. 12, No. 9, 6221942, 2012, p. 2735-2736.

Research output: Contribution to journalArticle

Stemple, C. Christopher ; Kwon, Hyuck Jin ; Yoon, Jeong-Yeol. / Rapid and sensitive detection of malaria antigen in human blood with lab-on-a-chip. In: IEEE Sensors Journal. 2012 ; Vol. 12, No. 9. pp. 2735-2736.
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