Rapid and sensitive detection of malaria antigen in human blood with lab-on-a-chip

C. Christopher Stemple, Hyuck Jin Kwon, Jeong Yeol Yoon

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A novel lab-on-a-chip detection device, based on the properties of immunoagglutination, was modified to detect malaria in the human blood through histidine-rich protein 2, an antigen expressed only by Plasmodium falciparum. Utilizing Mie scattering detection, which is angle- and size-dependent, the extent of immunoagglutination could be accurately measured while the optical disturbance from the human blood was minimized. The presence of human serum albumin in the blood is believed to further stabilize the antibody-conjugated submicron beads and/or break off the larger agglutinated beads. The lowest detection limit was 1 pg/mL in 10% of whole blood (equivalent to 10 pg/mL in undiluted whole blood), a few orders of magnitude lower than other assays. The final device is compact, with a fast assay time of approximately 8 min.

Original languageEnglish (US)
Article number6221942
Pages (from-to)2735-2736
Number of pages2
JournalIEEE Sensors Journal
Volume12
Issue number9
DOIs
StatePublished - Aug 9 2012

    Fingerprint

Keywords

  • Latex immunoagglutination
  • Mie scatter
  • microfluidics
  • plasmodium falciparum

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

Cite this