Rapid construction of capsid-modified adenoviral vectors through bacteriophage λ red recombination

Samuel K. Campos, Michael A. Barry

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

There are extensive efforts to develop cell-targeting adenoviral vectors for gene therapy wherein endogenous cell-binding ligands are ablated and exogenous ligands are introduced by genetic means. Although current approaches can genetically manipulate the capsid genes of adenoviral vectors, these approaches can be time-consuming and require multiple steps to produce a modified viral genome. We present here the use of the bacteriophage λ Red recombination system as a valuable tool for the easy and rapid construction of capsid-modified adenoviral genomes.

Original languageEnglish (US)
Pages (from-to)1125-1130
Number of pages6
JournalHuman Gene Therapy
Volume15
Issue number11
DOIs
StatePublished - Nov 2004

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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