Rapid construction of capsid-modified adenoviral vectors through bacteriophage λ red recombination

Samuel K Campos, Michael A. Barry

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

There are extensive efforts to develop cell-targeting adenoviral vectors for gene therapy wherein endogenous cell-binding ligands are ablated and exogenous ligands are introduced by genetic means. Although current approaches can genetically manipulate the capsid genes of adenoviral vectors, these approaches can be time-consuming and require multiple steps to produce a modified viral genome. We present here the use of the bacteriophage λ Red recombination system as a valuable tool for the easy and rapid construction of capsid-modified adenoviral genomes.

Original languageEnglish (US)
Pages (from-to)1125-1130
Number of pages6
JournalHuman Gene Therapy
Volume15
Issue number11
DOIs
StatePublished - Nov 2004
Externally publishedYes

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Capsid
Bacteriophages
Genetic Recombination
Ligands
Viral Genome
Genetic Therapy
Genome
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Rapid construction of capsid-modified adenoviral vectors through bacteriophage λ red recombination. / Campos, Samuel K; Barry, Michael A.

In: Human Gene Therapy, Vol. 15, No. 11, 11.2004, p. 1125-1130.

Research output: Contribution to journalArticle

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