Rapid flow cytometric analysis of the cell cycle in intact plant tissues

David W. Galbraith, Kristi R. Harkins, Joyce M. Maddox, Nicola M. Ayres, Dharam P. Sharma, Ebrahim Firoozabady

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Abstract

Mechanical chopping of plant tissues in the presence of mithramycin released intact nuclei representative of the cells within the tissues. The amount of nuclear DNA in the homogenates of monocotyledonous and dicotyledonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.

Original languageEnglish (US)
Pages (from-to)1049-1051
Number of pages3
JournalScience
Volume220
Issue number4601
DOIs
StatePublished - Jan 1 1983

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Galbraith, D. W., Harkins, K. R., Maddox, J. M., Ayres, N. M., Sharma, D. P., & Firoozabady, E. (1983). Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science, 220(4601), 1049-1051. https://doi.org/10.1126/science.220.4601.1049