Rapid fucosylation of intestinal epithelium sustains host-commensal symbiosis in sickness

Joseph M. Pickard, Corinne F. Maurice, Melissa A. Kinnebrew, Michael C. Abt, Dominik Schenten, Tatyana V. Golovkina, Said R. Bogatyrev, Rustem F. Ismagilov, Eric G. Pamer, Peter J. Turnbaugh, Alexander V. Chervonsky

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

Systemic infection induces conserved physiological responses that include both resistance and 'tolerance of infection' mechanisms. Temporary anorexia associated with an infection is often beneficial, reallocating energy from food foraging towards resistance to infection or depriving pathogens of nutrients. However, it imposes a stress on intestinal commensals, as they also experience reduced substrate availability; this affects host fitness owing to the loss of caloric intake and colonization resistance (protection from additional infections). We hypothesized that the host might utilize internal resources to support the gut microbiota during the acute phase of the disease. Here we show that systemic exposure to Toll-like receptor (TLR) ligands causes rapid α (1,2)-fucosylation of small intestine epithelial cells (IECs) in mice, which requires the sensing of TLR agonists, as well as the production of interleukin (IL)-23 by dendritic cells, activation of innate lymphoid cells and expression of fucosyltransferase 2 (Fut2) by IL-22-stimulated IECs. Fucosylated proteins are shed into the lumen and fucose is liberated and metabolized by the gut microbiota, as shown by reporter bacteria and community-wide analysis of microbial gene expression. Fucose affects the expression of microbial metabolic pathways and reduces the expression of bacterial virulence genes. It also improves host tolerance of the mild pathogen Citrobacter rodentium. Thus, rapid IEC fucosylation appears to be a protective mechanism that utilizes the host's resources to maintain host-microbial interactions during pathogen-induced stress.

Original languageEnglish (US)
Pages (from-to)638-641
Number of pages4
JournalNature
Volume514
Issue number7524
DOIs
StatePublished - Oct 1 2014

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Symbiosis
Intestinal Mucosa
Infection
Fucose
Toll-Like Receptors
Epithelial Cells
Intestines
Citrobacter rodentium
Microbial Genes
Microbial Interactions
Interleukin-23
Bacterial Genes
Food
Anorexia
Acute Disease
Metabolic Networks and Pathways
Energy Intake
Dendritic Cells
Small Intestine
Virulence

ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

Pickard, J. M., Maurice, C. F., Kinnebrew, M. A., Abt, M. C., Schenten, D., Golovkina, T. V., ... Chervonsky, A. V. (2014). Rapid fucosylation of intestinal epithelium sustains host-commensal symbiosis in sickness. Nature, 514(7524), 638-641. https://doi.org/10.1038/nature13823

Rapid fucosylation of intestinal epithelium sustains host-commensal symbiosis in sickness. / Pickard, Joseph M.; Maurice, Corinne F.; Kinnebrew, Melissa A.; Abt, Michael C.; Schenten, Dominik; Golovkina, Tatyana V.; Bogatyrev, Said R.; Ismagilov, Rustem F.; Pamer, Eric G.; Turnbaugh, Peter J.; Chervonsky, Alexander V.

In: Nature, Vol. 514, No. 7524, 01.10.2014, p. 638-641.

Research output: Contribution to journalArticle

Pickard, JM, Maurice, CF, Kinnebrew, MA, Abt, MC, Schenten, D, Golovkina, TV, Bogatyrev, SR, Ismagilov, RF, Pamer, EG, Turnbaugh, PJ & Chervonsky, AV 2014, 'Rapid fucosylation of intestinal epithelium sustains host-commensal symbiosis in sickness', Nature, vol. 514, no. 7524, pp. 638-641. https://doi.org/10.1038/nature13823
Pickard JM, Maurice CF, Kinnebrew MA, Abt MC, Schenten D, Golovkina TV et al. Rapid fucosylation of intestinal epithelium sustains host-commensal symbiosis in sickness. Nature. 2014 Oct 1;514(7524):638-641. https://doi.org/10.1038/nature13823
Pickard, Joseph M. ; Maurice, Corinne F. ; Kinnebrew, Melissa A. ; Abt, Michael C. ; Schenten, Dominik ; Golovkina, Tatyana V. ; Bogatyrev, Said R. ; Ismagilov, Rustem F. ; Pamer, Eric G. ; Turnbaugh, Peter J. ; Chervonsky, Alexander V. / Rapid fucosylation of intestinal epithelium sustains host-commensal symbiosis in sickness. In: Nature. 2014 ; Vol. 514, No. 7524. pp. 638-641.
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