Rapid Loss of Stress Fibers in Chinese Hamster Ovary Cells after Hyperthermia

James R. Glass, Robert G. DeWitt, Anne E. Cress

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

This study was initiated to characterize the effect of hyperthermia (45°) on the distribution of actin stress fibers in Chinese hamster ovary cells using rhodamine-conjugated phalloidin, a probe specific for F-actin. Fluorescent microscopy revealed a rapid loss of stress fibers after immersion in a 45° water bath. After 5-min immersion at 45°, approximately 90% of the cells analyzed did not contain observable stress fibers. Stress fibers were visible after incubation of cells at 37° after heating. The recovery of the appearance of the stress fibers occurred as protein synthesis resumed, and addition of protein synthesis inhibitors following heat treatment blocked the reappearance of these structures. These results support the hypothesis that cytoskeletal components may be a target of hyperthermia, explaining the pleotropic biological effects of heat and, in particular, heat radiosensitization.

Original languageEnglish (US)
Pages (from-to)258-262
Number of pages5
JournalCancer Research
Volume45
Issue number1
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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