Rapid sample preparation for determination of iron in tissues by closed-vessel digestion and microwave energy

D. B. Van Wyck, Ronald B Schifman, J. C. Stivelman, Joaquin Ruiz, D. Martin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We developed a rapid acid-digestion method for preparing tissue samples for iron determination. Specimens were digested in nitric acid and hydrogen peroxide under high temperature and pressure in closed Teflon vessels, with microwave energy. Analysis for iron in 25- to 250-mg portions of digested bovine liver powder (National Bureau of Standards Certified Reference Material no. 1577a) showed excellent linearity ([predicted] = 1.007[actual] - 0.166 μg per sample) and analytical recovery (98%). Precision (CV) was 5.4% when iron content was 10 μg per sample. Assaying split samples of mouse tissues, we found a close correlation between iron concentrations obtained with closed vs open vessels ([closed] = 0.878[open] + 68 μg/g, r = 0.994, range 400-4600 μg/g dry weight). In contrast to time-consuming conventional procedures for tissue dissolution, closed-vessel digestion with microwave energy dramatically shortens time for tissue preparation, minimizes use of caustic acid, reduces risk of sample loss or contamination, and yields accurate and reproducible results.

Original languageEnglish (US)
Pages (from-to)1128-1130
Number of pages3
JournalClinical Chemistry
Volume34
Issue number6
StatePublished - 1988
Externally publishedYes

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Microwaves
Digestion
Iron
Tissue
Nitric Acid
Caustics
Acids
Polytetrafluoroethylene
Liver
Powders
Hydrogen Peroxide
Dissolution
Contamination
Pressure
Weights and Measures
Recovery
Temperature

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Rapid sample preparation for determination of iron in tissues by closed-vessel digestion and microwave energy. / Van Wyck, D. B.; Schifman, Ronald B; Stivelman, J. C.; Ruiz, Joaquin; Martin, D.

In: Clinical Chemistry, Vol. 34, No. 6, 1988, p. 1128-1130.

Research output: Contribution to journalArticle

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N2 - We developed a rapid acid-digestion method for preparing tissue samples for iron determination. Specimens were digested in nitric acid and hydrogen peroxide under high temperature and pressure in closed Teflon vessels, with microwave energy. Analysis for iron in 25- to 250-mg portions of digested bovine liver powder (National Bureau of Standards Certified Reference Material no. 1577a) showed excellent linearity ([predicted] = 1.007[actual] - 0.166 μg per sample) and analytical recovery (98%). Precision (CV) was 5.4% when iron content was 10 μg per sample. Assaying split samples of mouse tissues, we found a close correlation between iron concentrations obtained with closed vs open vessels ([closed] = 0.878[open] + 68 μg/g, r = 0.994, range 400-4600 μg/g dry weight). In contrast to time-consuming conventional procedures for tissue dissolution, closed-vessel digestion with microwave energy dramatically shortens time for tissue preparation, minimizes use of caustic acid, reduces risk of sample loss or contamination, and yields accurate and reproducible results.

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