Rat muscle protein turnover and redox state in progressive diabetes

Julie M. Fagan, Soisungwan Satarug, Paul Cook, Marc E Tischler

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Protein synthesis and degradation, and redox state were measured in soleus and extensor digitorum longus muscles of rats up to 12 days after injection of streptozotocin. Muscle growth was slower in these animals apparently due to slower protein synthesis throughout the duration of diabetes. Up to day 4 after injection of streptozotocin or withdrawal of insulin from treated, diabetic animals, the muscle ratio of lactate/pyruvate, an indicator of the cytoplasmic NAD+ redox couple, was lower and protein degradation was faster than in control muscles. Thereafter, the ratio of lactate/pyruvate was greater and protein degradation was slower than in size- or age-matched control muscles. Insulin treatment in vitro or in vivo increased lactate/pyruvate and decreased proteolysis. Therefore, in muscles of streptozotocin-diabetic rats, the initial increase and later fall in proteolysis, and the inhibition of proteolysis by insulin, may correlate with opposite changes in NADH/NAD+.

Original languageEnglish (US)
Pages (from-to)783-790
Number of pages8
JournalLife Sciences
Volume40
Issue number8
DOIs
StatePublished - Feb 23 1987

Fingerprint

Muscle Proteins
Medical problems
Proteolysis
Oxidation-Reduction
Muscle
Rats
Muscles
Streptozocin
Pyruvic Acid
NAD
Lactic Acid
Insulin
Degradation
Animals
Proteins
Enzyme inhibition
Injections
Growth

ASJC Scopus subject areas

  • Pharmacology

Cite this

Rat muscle protein turnover and redox state in progressive diabetes. / Fagan, Julie M.; Satarug, Soisungwan; Cook, Paul; Tischler, Marc E.

In: Life Sciences, Vol. 40, No. 8, 23.02.1987, p. 783-790.

Research output: Contribution to journalArticle

Fagan, Julie M. ; Satarug, Soisungwan ; Cook, Paul ; Tischler, Marc E. / Rat muscle protein turnover and redox state in progressive diabetes. In: Life Sciences. 1987 ; Vol. 40, No. 8. pp. 783-790.
@article{18704499cf0e4309956f37cab7a28a1b,
title = "Rat muscle protein turnover and redox state in progressive diabetes",
abstract = "Protein synthesis and degradation, and redox state were measured in soleus and extensor digitorum longus muscles of rats up to 12 days after injection of streptozotocin. Muscle growth was slower in these animals apparently due to slower protein synthesis throughout the duration of diabetes. Up to day 4 after injection of streptozotocin or withdrawal of insulin from treated, diabetic animals, the muscle ratio of lactate/pyruvate, an indicator of the cytoplasmic NAD+ redox couple, was lower and protein degradation was faster than in control muscles. Thereafter, the ratio of lactate/pyruvate was greater and protein degradation was slower than in size- or age-matched control muscles. Insulin treatment in vitro or in vivo increased lactate/pyruvate and decreased proteolysis. Therefore, in muscles of streptozotocin-diabetic rats, the initial increase and later fall in proteolysis, and the inhibition of proteolysis by insulin, may correlate with opposite changes in NADH/NAD+.",
author = "Fagan, {Julie M.} and Soisungwan Satarug and Paul Cook and Tischler, {Marc E}",
year = "1987",
month = "2",
day = "23",
doi = "10.1016/0024-3205(87)90306-7",
language = "English (US)",
volume = "40",
pages = "783--790",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "8",

}

TY - JOUR

T1 - Rat muscle protein turnover and redox state in progressive diabetes

AU - Fagan, Julie M.

AU - Satarug, Soisungwan

AU - Cook, Paul

AU - Tischler, Marc E

PY - 1987/2/23

Y1 - 1987/2/23

N2 - Protein synthesis and degradation, and redox state were measured in soleus and extensor digitorum longus muscles of rats up to 12 days after injection of streptozotocin. Muscle growth was slower in these animals apparently due to slower protein synthesis throughout the duration of diabetes. Up to day 4 after injection of streptozotocin or withdrawal of insulin from treated, diabetic animals, the muscle ratio of lactate/pyruvate, an indicator of the cytoplasmic NAD+ redox couple, was lower and protein degradation was faster than in control muscles. Thereafter, the ratio of lactate/pyruvate was greater and protein degradation was slower than in size- or age-matched control muscles. Insulin treatment in vitro or in vivo increased lactate/pyruvate and decreased proteolysis. Therefore, in muscles of streptozotocin-diabetic rats, the initial increase and later fall in proteolysis, and the inhibition of proteolysis by insulin, may correlate with opposite changes in NADH/NAD+.

AB - Protein synthesis and degradation, and redox state were measured in soleus and extensor digitorum longus muscles of rats up to 12 days after injection of streptozotocin. Muscle growth was slower in these animals apparently due to slower protein synthesis throughout the duration of diabetes. Up to day 4 after injection of streptozotocin or withdrawal of insulin from treated, diabetic animals, the muscle ratio of lactate/pyruvate, an indicator of the cytoplasmic NAD+ redox couple, was lower and protein degradation was faster than in control muscles. Thereafter, the ratio of lactate/pyruvate was greater and protein degradation was slower than in size- or age-matched control muscles. Insulin treatment in vitro or in vivo increased lactate/pyruvate and decreased proteolysis. Therefore, in muscles of streptozotocin-diabetic rats, the initial increase and later fall in proteolysis, and the inhibition of proteolysis by insulin, may correlate with opposite changes in NADH/NAD+.

UR - http://www.scopus.com/inward/record.url?scp=0023122440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023122440&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(87)90306-7

DO - 10.1016/0024-3205(87)90306-7

M3 - Article

VL - 40

SP - 783

EP - 790

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 8

ER -