Reagents for developmental regulation of Hedgehog signaling

Cristy Lewis, Paul A. Krieg

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We have examined a number of reagents for their ability to modulate activity of the Hh signaling pathway during embryonic development of Xenopus. In particular we have focused on regulation of events occurring during tailbud stages and later. Two inducible protein reagents based on the Gli1 and Gli3 transcription factors were generated and the activity of these proteins was compared to the Hh signaling pathway inhibitor, cyclopamine, and the activators, Smoothened agonist (SAG) and purmorphamine (PMA). Effectiveness of reagents was assayed using both molecular biological techniques and biological readouts. We found that the small molecule modulators of the Hh pathway were highly specific and effective and produced results generally superior to the more conventional protein reagents for examination of later stage developmental processes.

Original languageEnglish (US)
Pages (from-to)390-397
Number of pages8
JournalMethods
Volume66
Issue number3
DOIs
StatePublished - Apr 1 2014

Keywords

  • Cyclopamine
  • Dorsal aorta
  • Gli1
  • Gli3
  • Hedgehog
  • Purmorphamine (PMA)
  • Smoothened agonist (SAG)

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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