Real-time quantitative RT-PCR of cyclin D1 mRNA in mantle cell lymphoma: Comparison with FISH and immunohistochemistry

Pei Hui, John G. Howe, Jill Crouch, Manjunath Nimmakayalu, Mazin B. Qumsiyeh, Giovanni Tallini, Stuart D Flynn, Brian R. Smith

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Presence of the balanced translocation t(11;14)(q13;q32) and the consequent overexpression of cyclin D1 found in mantle cell lymphoma (MCL) has been shown to be of important diagnostic value. Although many molecular and immunohistochemical approaches have been applied to analyze cyclin D1 status, correlative studies to compare different methods for the diagnosis of MCL are lacking. In this study, we examined 39 archived paraffin specimens from patients diagnosed with a variety of lymphoproliferative diseases including nine cases meeting morphologic and immunophenotypic criteria for MCL by: (1) real-time quantitative RT-PCR to evaluate cyclin D1 mRNA expression; (2) dual fluorescence in situ hybridization (FISH) to evaluate the t(l1;14) translocation in interphase nuclei; and (3) tissue array immunohistochemistry to evaluate the cyclin D1 protein level. Among the nine cases of possible MCL, seven cases showed overexpression of cyclin D1 mRNA (cyclin D1 positive MCL) and two cases showed no cyclin D1 mRNA increase (cyclin D1 negative "MCL-like"). In six of seven cyclin D1 positive cases, the t(11;14) translocation was demonstrated by FISH analysis; in one case FISH was unsuccessful. Six of the seven cyclin D1 mRNA overexpressing cases showed increased cyclin D1 protein on tissue array immunohistochemistry; one was technically suboptimal. Among the two cyclin D1 negative MCL-like cases, FISH confirmed the absence of the t(11;14) translocation in both cases. All other lymphoproliferative diseases studied were found to have low or no cyclin D1 mRNA expression and were easily distinguishable from the cyclin D1 overexpressing MCLs by all three techniques. In addition, to confirming the need to assess cyclin D1 status, as well as, morphology and immunophenotyping to establish the diagnosis of MCL, this study demonstrates good correlation and comparability between measure of cyclin D1 mRNA, the 11;14 translocation and cyclin D1 protein.

Original languageEnglish (US)
Pages (from-to)1385-1394
Number of pages10
JournalLeukemia and Lymphoma
Volume44
Issue number8
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Fingerprint

Mantle-Cell Lymphoma
Cyclin D1
Fluorescence In Situ Hybridization
Real-Time Polymerase Chain Reaction
Immunohistochemistry
Messenger RNA
Immunophenotyping
Proteins
Interphase

Keywords

  • Cylin D1
  • Mantle cell lymphoma
  • Real-time quantitative RT-PCR
  • Tissue array

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Hui, P., Howe, J. G., Crouch, J., Nimmakayalu, M., Qumsiyeh, M. B., Tallini, G., ... Smith, B. R. (2003). Real-time quantitative RT-PCR of cyclin D1 mRNA in mantle cell lymphoma: Comparison with FISH and immunohistochemistry. Leukemia and Lymphoma, 44(8), 1385-1394. https://doi.org/10.1080/1042819031000079168

Real-time quantitative RT-PCR of cyclin D1 mRNA in mantle cell lymphoma : Comparison with FISH and immunohistochemistry. / Hui, Pei; Howe, John G.; Crouch, Jill; Nimmakayalu, Manjunath; Qumsiyeh, Mazin B.; Tallini, Giovanni; Flynn, Stuart D; Smith, Brian R.

In: Leukemia and Lymphoma, Vol. 44, No. 8, 01.08.2003, p. 1385-1394.

Research output: Contribution to journalArticle

Hui, Pei ; Howe, John G. ; Crouch, Jill ; Nimmakayalu, Manjunath ; Qumsiyeh, Mazin B. ; Tallini, Giovanni ; Flynn, Stuart D ; Smith, Brian R. / Real-time quantitative RT-PCR of cyclin D1 mRNA in mantle cell lymphoma : Comparison with FISH and immunohistochemistry. In: Leukemia and Lymphoma. 2003 ; Vol. 44, No. 8. pp. 1385-1394.
@article{9bc428327d23448fb9ebd4e0d09c08de,
title = "Real-time quantitative RT-PCR of cyclin D1 mRNA in mantle cell lymphoma: Comparison with FISH and immunohistochemistry",
abstract = "Presence of the balanced translocation t(11;14)(q13;q32) and the consequent overexpression of cyclin D1 found in mantle cell lymphoma (MCL) has been shown to be of important diagnostic value. Although many molecular and immunohistochemical approaches have been applied to analyze cyclin D1 status, correlative studies to compare different methods for the diagnosis of MCL are lacking. In this study, we examined 39 archived paraffin specimens from patients diagnosed with a variety of lymphoproliferative diseases including nine cases meeting morphologic and immunophenotypic criteria for MCL by: (1) real-time quantitative RT-PCR to evaluate cyclin D1 mRNA expression; (2) dual fluorescence in situ hybridization (FISH) to evaluate the t(l1;14) translocation in interphase nuclei; and (3) tissue array immunohistochemistry to evaluate the cyclin D1 protein level. Among the nine cases of possible MCL, seven cases showed overexpression of cyclin D1 mRNA (cyclin D1 positive MCL) and two cases showed no cyclin D1 mRNA increase (cyclin D1 negative {"}MCL-like{"}). In six of seven cyclin D1 positive cases, the t(11;14) translocation was demonstrated by FISH analysis; in one case FISH was unsuccessful. Six of the seven cyclin D1 mRNA overexpressing cases showed increased cyclin D1 protein on tissue array immunohistochemistry; one was technically suboptimal. Among the two cyclin D1 negative MCL-like cases, FISH confirmed the absence of the t(11;14) translocation in both cases. All other lymphoproliferative diseases studied were found to have low or no cyclin D1 mRNA expression and were easily distinguishable from the cyclin D1 overexpressing MCLs by all three techniques. In addition, to confirming the need to assess cyclin D1 status, as well as, morphology and immunophenotyping to establish the diagnosis of MCL, this study demonstrates good correlation and comparability between measure of cyclin D1 mRNA, the 11;14 translocation and cyclin D1 protein.",
keywords = "Cylin D1, Mantle cell lymphoma, Real-time quantitative RT-PCR, Tissue array",
author = "Pei Hui and Howe, {John G.} and Jill Crouch and Manjunath Nimmakayalu and Qumsiyeh, {Mazin B.} and Giovanni Tallini and Flynn, {Stuart D} and Smith, {Brian R.}",
year = "2003",
month = "8",
day = "1",
doi = "10.1080/1042819031000079168",
language = "English (US)",
volume = "44",
pages = "1385--1394",
journal = "Leukemia and Lymphoma",
issn = "1042-8194",
publisher = "Informa Healthcare",
number = "8",

}

TY - JOUR

T1 - Real-time quantitative RT-PCR of cyclin D1 mRNA in mantle cell lymphoma

T2 - Comparison with FISH and immunohistochemistry

AU - Hui, Pei

AU - Howe, John G.

AU - Crouch, Jill

AU - Nimmakayalu, Manjunath

AU - Qumsiyeh, Mazin B.

AU - Tallini, Giovanni

AU - Flynn, Stuart D

AU - Smith, Brian R.

PY - 2003/8/1

Y1 - 2003/8/1

N2 - Presence of the balanced translocation t(11;14)(q13;q32) and the consequent overexpression of cyclin D1 found in mantle cell lymphoma (MCL) has been shown to be of important diagnostic value. Although many molecular and immunohistochemical approaches have been applied to analyze cyclin D1 status, correlative studies to compare different methods for the diagnosis of MCL are lacking. In this study, we examined 39 archived paraffin specimens from patients diagnosed with a variety of lymphoproliferative diseases including nine cases meeting morphologic and immunophenotypic criteria for MCL by: (1) real-time quantitative RT-PCR to evaluate cyclin D1 mRNA expression; (2) dual fluorescence in situ hybridization (FISH) to evaluate the t(l1;14) translocation in interphase nuclei; and (3) tissue array immunohistochemistry to evaluate the cyclin D1 protein level. Among the nine cases of possible MCL, seven cases showed overexpression of cyclin D1 mRNA (cyclin D1 positive MCL) and two cases showed no cyclin D1 mRNA increase (cyclin D1 negative "MCL-like"). In six of seven cyclin D1 positive cases, the t(11;14) translocation was demonstrated by FISH analysis; in one case FISH was unsuccessful. Six of the seven cyclin D1 mRNA overexpressing cases showed increased cyclin D1 protein on tissue array immunohistochemistry; one was technically suboptimal. Among the two cyclin D1 negative MCL-like cases, FISH confirmed the absence of the t(11;14) translocation in both cases. All other lymphoproliferative diseases studied were found to have low or no cyclin D1 mRNA expression and were easily distinguishable from the cyclin D1 overexpressing MCLs by all three techniques. In addition, to confirming the need to assess cyclin D1 status, as well as, morphology and immunophenotyping to establish the diagnosis of MCL, this study demonstrates good correlation and comparability between measure of cyclin D1 mRNA, the 11;14 translocation and cyclin D1 protein.

AB - Presence of the balanced translocation t(11;14)(q13;q32) and the consequent overexpression of cyclin D1 found in mantle cell lymphoma (MCL) has been shown to be of important diagnostic value. Although many molecular and immunohistochemical approaches have been applied to analyze cyclin D1 status, correlative studies to compare different methods for the diagnosis of MCL are lacking. In this study, we examined 39 archived paraffin specimens from patients diagnosed with a variety of lymphoproliferative diseases including nine cases meeting morphologic and immunophenotypic criteria for MCL by: (1) real-time quantitative RT-PCR to evaluate cyclin D1 mRNA expression; (2) dual fluorescence in situ hybridization (FISH) to evaluate the t(l1;14) translocation in interphase nuclei; and (3) tissue array immunohistochemistry to evaluate the cyclin D1 protein level. Among the nine cases of possible MCL, seven cases showed overexpression of cyclin D1 mRNA (cyclin D1 positive MCL) and two cases showed no cyclin D1 mRNA increase (cyclin D1 negative "MCL-like"). In six of seven cyclin D1 positive cases, the t(11;14) translocation was demonstrated by FISH analysis; in one case FISH was unsuccessful. Six of the seven cyclin D1 mRNA overexpressing cases showed increased cyclin D1 protein on tissue array immunohistochemistry; one was technically suboptimal. Among the two cyclin D1 negative MCL-like cases, FISH confirmed the absence of the t(11;14) translocation in both cases. All other lymphoproliferative diseases studied were found to have low or no cyclin D1 mRNA expression and were easily distinguishable from the cyclin D1 overexpressing MCLs by all three techniques. In addition, to confirming the need to assess cyclin D1 status, as well as, morphology and immunophenotyping to establish the diagnosis of MCL, this study demonstrates good correlation and comparability between measure of cyclin D1 mRNA, the 11;14 translocation and cyclin D1 protein.

KW - Cylin D1

KW - Mantle cell lymphoma

KW - Real-time quantitative RT-PCR

KW - Tissue array

UR - http://www.scopus.com/inward/record.url?scp=0038466245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038466245&partnerID=8YFLogxK

U2 - 10.1080/1042819031000079168

DO - 10.1080/1042819031000079168

M3 - Article

C2 - 12952233

AN - SCOPUS:0038466245

VL - 44

SP - 1385

EP - 1394

JO - Leukemia and Lymphoma

JF - Leukemia and Lymphoma

SN - 1042-8194

IS - 8

ER -