Recent developments in microarray-based enzyme assays: From functional annotation to substrate/inhibitor fingerprinting

Hongyan Sun, Souvik Chattopadhaya, Jun Wang, Shao Q. Yao

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Recent advances in proteomics have provided impetus towards the development of robust technologies for high-throughput studies of enzymes. The term "catalomics" defines an emerging '-omics' field in which high-throughput studies of enzymes are carried out by using advanced chemical proteomics approaches. Of the various available methods, microarrays have emerged as a powerful and versatile platform to accelerate not only the functional annotation but also the substrate and inhibitor specificity (e.g. substrate and inhibitor fingerprinting, respectively) of enzymes. Herein, we review recent developments in the fabrication of various types of microarray technologies (protein-, peptide- and small-molecule-based microarrays) and their applications in high-throughput characterizations of enzymes.

Original languageEnglish (US)
Pages (from-to)416-426
Number of pages11
JournalAnalytical and bioanalytical chemistry
Volume386
Issue number3
DOIs
StatePublished - Oct 1 2006
Externally publishedYes

Keywords

  • Activity-based fingerprinting
  • Activity-based probe
  • Catalomics
  • Drug discovery
  • Enzyme assay
  • Inhibitor fingerprinting
  • Microarray

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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