Receptor binding sites for atrial natriuretic factor are expressed by brown adipose tissue

Angelito C. Bacay, Christopher R. Mantyh, Steven R. Vigna, Patrick W Mantyh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

To explore the possibility that atrial natriuretic factor (ANF) is involved in thermoregulation we used quantiative receptor autoradiography and homogenate receptor binding assays to identify ANF bindings sites in neonatal rat and sheep brown adipose tissue, respectively. Using quantitative receptor autoradiography were were able to localize high levels of specific binding sites for 125I-rat ANF in neonatal rat brown adipose tissue. Homogenate binding assays on sheep brown fat demonstrated that the radioligand was binding to the membrane fraction and that the specific binding was not due to a lipophilic interaction between 125I-rat ANF and brown fat. Specific binding of 125I-rat ANF to the membranes of brown fat cells was inhibited by unlabeled rat ANF with a K1 of 8.0×10-9M, but not by unrelated peptides. These studies demonstrate that brown fat cells express high levels of ANF receptor binding sites in neonatal rat and sheep and suggest that ANF may play a role in thermoregulation.

Original languageEnglish (US)
Pages (from-to)1021-1026
Number of pages6
JournalPeptides
Volume9
Issue number5
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Brown Adipose Tissue
Atrial Natriuretic Factor
Fats
Binding Sites
Brown Adipocytes
Tissue
Rats
Sheep
Body Temperature Regulation
Autoradiography
Assays
Atrial Natriuretic Factor Receptors
Membranes
Peptides
rat atrial natriuretic peptide

Keywords

  • Atrial natriuretic factor
  • Brown adipose tissue
  • Sympathetic nervous system
  • Thermoregulation

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Receptor binding sites for atrial natriuretic factor are expressed by brown adipose tissue. / Bacay, Angelito C.; Mantyh, Christopher R.; Vigna, Steven R.; Mantyh, Patrick W.

In: Peptides, Vol. 9, No. 5, 1988, p. 1021-1026.

Research output: Contribution to journalArticle

Bacay, Angelito C. ; Mantyh, Christopher R. ; Vigna, Steven R. ; Mantyh, Patrick W. / Receptor binding sites for atrial natriuretic factor are expressed by brown adipose tissue. In: Peptides. 1988 ; Vol. 9, No. 5. pp. 1021-1026.
@article{824399e379db412b9099aab851cc647f,
title = "Receptor binding sites for atrial natriuretic factor are expressed by brown adipose tissue",
abstract = "To explore the possibility that atrial natriuretic factor (ANF) is involved in thermoregulation we used quantiative receptor autoradiography and homogenate receptor binding assays to identify ANF bindings sites in neonatal rat and sheep brown adipose tissue, respectively. Using quantitative receptor autoradiography were were able to localize high levels of specific binding sites for 125I-rat ANF in neonatal rat brown adipose tissue. Homogenate binding assays on sheep brown fat demonstrated that the radioligand was binding to the membrane fraction and that the specific binding was not due to a lipophilic interaction between 125I-rat ANF and brown fat. Specific binding of 125I-rat ANF to the membranes of brown fat cells was inhibited by unlabeled rat ANF with a K1 of 8.0×10-9M, but not by unrelated peptides. These studies demonstrate that brown fat cells express high levels of ANF receptor binding sites in neonatal rat and sheep and suggest that ANF may play a role in thermoregulation.",
keywords = "Atrial natriuretic factor, Brown adipose tissue, Sympathetic nervous system, Thermoregulation",
author = "Bacay, {Angelito C.} and Mantyh, {Christopher R.} and Vigna, {Steven R.} and Mantyh, {Patrick W}",
year = "1988",
doi = "10.1016/0196-9781(88)90083-6",
language = "English (US)",
volume = "9",
pages = "1021--1026",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Receptor binding sites for atrial natriuretic factor are expressed by brown adipose tissue

AU - Bacay, Angelito C.

AU - Mantyh, Christopher R.

AU - Vigna, Steven R.

AU - Mantyh, Patrick W

PY - 1988

Y1 - 1988

N2 - To explore the possibility that atrial natriuretic factor (ANF) is involved in thermoregulation we used quantiative receptor autoradiography and homogenate receptor binding assays to identify ANF bindings sites in neonatal rat and sheep brown adipose tissue, respectively. Using quantitative receptor autoradiography were were able to localize high levels of specific binding sites for 125I-rat ANF in neonatal rat brown adipose tissue. Homogenate binding assays on sheep brown fat demonstrated that the radioligand was binding to the membrane fraction and that the specific binding was not due to a lipophilic interaction between 125I-rat ANF and brown fat. Specific binding of 125I-rat ANF to the membranes of brown fat cells was inhibited by unlabeled rat ANF with a K1 of 8.0×10-9M, but not by unrelated peptides. These studies demonstrate that brown fat cells express high levels of ANF receptor binding sites in neonatal rat and sheep and suggest that ANF may play a role in thermoregulation.

AB - To explore the possibility that atrial natriuretic factor (ANF) is involved in thermoregulation we used quantiative receptor autoradiography and homogenate receptor binding assays to identify ANF bindings sites in neonatal rat and sheep brown adipose tissue, respectively. Using quantitative receptor autoradiography were were able to localize high levels of specific binding sites for 125I-rat ANF in neonatal rat brown adipose tissue. Homogenate binding assays on sheep brown fat demonstrated that the radioligand was binding to the membrane fraction and that the specific binding was not due to a lipophilic interaction between 125I-rat ANF and brown fat. Specific binding of 125I-rat ANF to the membranes of brown fat cells was inhibited by unlabeled rat ANF with a K1 of 8.0×10-9M, but not by unrelated peptides. These studies demonstrate that brown fat cells express high levels of ANF receptor binding sites in neonatal rat and sheep and suggest that ANF may play a role in thermoregulation.

KW - Atrial natriuretic factor

KW - Brown adipose tissue

KW - Sympathetic nervous system

KW - Thermoregulation

UR - http://www.scopus.com/inward/record.url?scp=0024152396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024152396&partnerID=8YFLogxK

U2 - 10.1016/0196-9781(88)90083-6

DO - 10.1016/0196-9781(88)90083-6

M3 - Article

C2 - 2854242

AN - SCOPUS:0024152396

VL - 9

SP - 1021

EP - 1026

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 5

ER -