Receptor for 1,25-Dihydroxyvitamin D3 in GH3 pituitary cells

Mark R Haussler, S. C. Manolagas, L. J. Deftos

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Cytosol prepared in 0.3 M KCl from pituitary GH3 cells, but not from AtT-20 cells contains a receptor-like macromolecule that binds 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) with specificity and high affinity (kd = 2.9 × 10-10 M). The GHD3 cytosolic binding component sediments at 3.3 S in high-salt sucrose gradients and adsorbs to DNA-cellulose; its elution profile from DNA-cellulose and other biochemical properties are indistinguishable from those of classical 1,25(OH)2D3 hormone receptors. The presence of the l,25(OH)2D3 receptor in pituitary cells which secrete primarily growth hormone and prolactin (GH3), but not in a line which secretes the 31,000-dalton ACTH precursor and its derived peptides (AtT-20), suggests that 1,25(OH)2D3 may play a regulatory role in specific pituitary cells.

Original languageEnglish (US)
Pages (from-to)15-19
Number of pages5
JournalJournal of Steroid Biochemistry
Volume16
Issue number1
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Calcitriol Receptors
Calcitriol
Macromolecules
Prolactin
Adrenocorticotropic Hormone
Growth Hormone
Sucrose
Sediments
Salts
Hormones
Peptides
Cytosol
DNA-cellulose

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Receptor for 1,25-Dihydroxyvitamin D3 in GH3 pituitary cells. / Haussler, Mark R; Manolagas, S. C.; Deftos, L. J.

In: Journal of Steroid Biochemistry, Vol. 16, No. 1, 1982, p. 15-19.

Research output: Contribution to journalArticle

Haussler, Mark R ; Manolagas, S. C. ; Deftos, L. J. / Receptor for 1,25-Dihydroxyvitamin D3 in GH3 pituitary cells. In: Journal of Steroid Biochemistry. 1982 ; Vol. 16, No. 1. pp. 15-19.
@article{58e61de18cab42eeac9810236f65408f,
title = "Receptor for 1,25-Dihydroxyvitamin D3 in GH3 pituitary cells",
abstract = "Cytosol prepared in 0.3 M KCl from pituitary GH3 cells, but not from AtT-20 cells contains a receptor-like macromolecule that binds 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) with specificity and high affinity (kd = 2.9 × 10-10 M). The GHD3 cytosolic binding component sediments at 3.3 S in high-salt sucrose gradients and adsorbs to DNA-cellulose; its elution profile from DNA-cellulose and other biochemical properties are indistinguishable from those of classical 1,25(OH)2D3 hormone receptors. The presence of the l,25(OH)2D3 receptor in pituitary cells which secrete primarily growth hormone and prolactin (GH3), but not in a line which secretes the 31,000-dalton ACTH precursor and its derived peptides (AtT-20), suggests that 1,25(OH)2D3 may play a regulatory role in specific pituitary cells.",
author = "Haussler, {Mark R} and Manolagas, {S. C.} and Deftos, {L. J.}",
year = "1982",
doi = "10.1016/0022-4731(82)90138-8",
language = "English (US)",
volume = "16",
pages = "15--19",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
issn = "0960-0760",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Receptor for 1,25-Dihydroxyvitamin D3 in GH3 pituitary cells

AU - Haussler, Mark R

AU - Manolagas, S. C.

AU - Deftos, L. J.

PY - 1982

Y1 - 1982

N2 - Cytosol prepared in 0.3 M KCl from pituitary GH3 cells, but not from AtT-20 cells contains a receptor-like macromolecule that binds 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) with specificity and high affinity (kd = 2.9 × 10-10 M). The GHD3 cytosolic binding component sediments at 3.3 S in high-salt sucrose gradients and adsorbs to DNA-cellulose; its elution profile from DNA-cellulose and other biochemical properties are indistinguishable from those of classical 1,25(OH)2D3 hormone receptors. The presence of the l,25(OH)2D3 receptor in pituitary cells which secrete primarily growth hormone and prolactin (GH3), but not in a line which secretes the 31,000-dalton ACTH precursor and its derived peptides (AtT-20), suggests that 1,25(OH)2D3 may play a regulatory role in specific pituitary cells.

AB - Cytosol prepared in 0.3 M KCl from pituitary GH3 cells, but not from AtT-20 cells contains a receptor-like macromolecule that binds 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) with specificity and high affinity (kd = 2.9 × 10-10 M). The GHD3 cytosolic binding component sediments at 3.3 S in high-salt sucrose gradients and adsorbs to DNA-cellulose; its elution profile from DNA-cellulose and other biochemical properties are indistinguishable from those of classical 1,25(OH)2D3 hormone receptors. The presence of the l,25(OH)2D3 receptor in pituitary cells which secrete primarily growth hormone and prolactin (GH3), but not in a line which secretes the 31,000-dalton ACTH precursor and its derived peptides (AtT-20), suggests that 1,25(OH)2D3 may play a regulatory role in specific pituitary cells.

UR - http://www.scopus.com/inward/record.url?scp=0020048029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020048029&partnerID=8YFLogxK

U2 - 10.1016/0022-4731(82)90138-8

DO - 10.1016/0022-4731(82)90138-8

M3 - Article

VL - 16

SP - 15

EP - 19

JO - Journal of Steroid Biochemistry and Molecular Biology

JF - Journal of Steroid Biochemistry and Molecular Biology

SN - 0960-0760

IS - 1

ER -