Recognition of a dominant epitope in bovine heat-shock protein 70 in inner ear disease

Donald B. Bloch, Jesus A. Gutierrez, Vincent Guerriero, Steven D. Rauch, Kurt J. Bloch

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objective: To investigate the specificity of antibodies to heat-shock protein 70 (HSP70) in patients with idiopathic, progressive, bilateral sensorineural hearing loss (IPBSNHL) and Meniere disease. Study Design: Test immunoreactivity of patients' sera using recombinant human (rh) and bovine (rb) HSP70, as well as segments representing different regions of bovine HSP70 as antigen. Methods: Sera were tested by Western blotting. Results: Of 52 patients with IPBSNHL, 40 sera reacted only with rbHSP70; 12 reacted with both rbHSP70 and rhHSP70. Sera from 13 patients with IPBSNHL and from 8 with Meniere disease were tested on the panel of bovine HSP70 segments. Eleven and 7 samples, respectively, reacted with amino acid segment 427-461 from the carboxy (C)-terminal region of the molecule. Conclusion: In IPBSNHL and Meniere disease, antibodies are directed primarily against an epitope(s) within the C-terminal region of HSP70 where diversity in sequence among different species, including possible pathogens, is greatest. These findings may provide clues to the pathogenesis or specific serodiagnosis (or both) of diseases of the inner ear.

Original languageEnglish (US)
Pages (from-to)621-625
Number of pages5
JournalLaryngoscope
Volume109
Issue number4
StatePublished - 1999

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Labyrinth Diseases
HSP70 Heat-Shock Proteins
Bilateral Hearing Loss
Sensorineural Hearing Loss
Epitopes
Meniere Disease
Serum
Antibody Specificity
Serologic Tests
Western Blotting
Antigens
Amino Acids
Antibodies

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Bloch, D. B., Gutierrez, J. A., Guerriero, V., Rauch, S. D., & Bloch, K. J. (1999). Recognition of a dominant epitope in bovine heat-shock protein 70 in inner ear disease. Laryngoscope, 109(4), 621-625.

Recognition of a dominant epitope in bovine heat-shock protein 70 in inner ear disease. / Bloch, Donald B.; Gutierrez, Jesus A.; Guerriero, Vincent; Rauch, Steven D.; Bloch, Kurt J.

In: Laryngoscope, Vol. 109, No. 4, 1999, p. 621-625.

Research output: Contribution to journalArticle

Bloch, DB, Gutierrez, JA, Guerriero, V, Rauch, SD & Bloch, KJ 1999, 'Recognition of a dominant epitope in bovine heat-shock protein 70 in inner ear disease', Laryngoscope, vol. 109, no. 4, pp. 621-625.
Bloch, Donald B. ; Gutierrez, Jesus A. ; Guerriero, Vincent ; Rauch, Steven D. ; Bloch, Kurt J. / Recognition of a dominant epitope in bovine heat-shock protein 70 in inner ear disease. In: Laryngoscope. 1999 ; Vol. 109, No. 4. pp. 621-625.
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