Recombinant silk-elastinlike protein polymer displays elasticity comparable to elastin

Weibing Teng, Joseph Cappello, Xiaoyi Wu

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We evaluated the mechanical properties of the genetically engineered, recombinant silk-elastinlike protein copolymer, SELP-47K. In tensile stress-strain analysis, methanol-treated non-cross-linked SELP-47K films exceeded the properties of native aortic elastin, attaining an ultimate tensile strength of 2.5 ± 0.4 MPa, an elastic modulus of 1.7 ± 0.4 MPa, an extensibility of 190 ± 60%, and a resilience of 86 ± 4% after 10 cycles of mechanical preconditioning. Stress-relaxation and creep analysis showed that films substantially maintained their elastic properties under sustained deformation. Chemical cross-linking of SELP-47K films doubled the elastic modulus and ultimate tensile strength and enhanced the extensibility and resilience. The underlying conformational and microstructural features of the films were examined. Raman spectroscopy revealed that the silklike blocks of SELP-47K existed in antiparallel β-sheet crystals in the films, likely responsible for the robust physical cross-links. Scanning electron microscopy (SEM) revealed that the various processing treatments and the mechanical deformation of the films induced changes in their surface microstructure consistent with the coagulation and alignment of polymer chains. These results demonstrate that films with excellent elasticity, comparable to native aortic elastin, are obtainable from SELP-47K, a protein copolymer combining both silk- and elastin-derived sequences in a single polymer chain.

Original languageEnglish (US)
Pages (from-to)3028-3036
Number of pages9
JournalBiomacromolecules
Volume10
Issue number11
DOIs
StatePublished - Nov 9 2009

Fingerprint

Elastin
Silk
Elasticity
Polymers
Proteins
Tensile strength
Copolymers
Elastic moduli
Stress relaxation
Coagulation
Tensile stress
Methanol
Raman spectroscopy
Creep
silk-elastinlike protein 47K
Mechanical properties
Crystals
Microstructure
Scanning electron microscopy
Processing

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

Cite this

Recombinant silk-elastinlike protein polymer displays elasticity comparable to elastin. / Teng, Weibing; Cappello, Joseph; Wu, Xiaoyi.

In: Biomacromolecules, Vol. 10, No. 11, 09.11.2009, p. 3028-3036.

Research output: Contribution to journalArticle

Teng, Weibing ; Cappello, Joseph ; Wu, Xiaoyi. / Recombinant silk-elastinlike protein polymer displays elasticity comparable to elastin. In: Biomacromolecules. 2009 ; Vol. 10, No. 11. pp. 3028-3036.
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