Reduction of Arcobacter at two conventional wastewater treatment plants in southern Arizona, USA

Rajani Ghaju Shrestha, Samendra P. Sherchan, Masaaki Kitajima, Yasuhiro Tanaka, Charles P. Gerba, Eiji Haramoto

Research output: Contribution to journalArticle

Abstract

This study aimed to identify the bacterial community in two wastewater treatment plants (WWTPs) and to determine the occurrence and reduction of Arcobacter, along with virulence genes (ciaB and pldA). A total of 48 samples (24 influent and 24 effluent) were collected at two WWTPs in southern Arizona in the United States, monthly from August 2011 to July 2012. Bacterial DNA extract was utilized for 16S rRNA metagenomic sequencing. Quantification of Arcobacter 16S rRNA gene was conducted using a recently developed SYBR Green-based quantitative PCR assay. Among 847 genera identified, 113 (13%) were identified as potentially pathogenic bacteria. Arcobacter 16S rRNA gene was detected in all influent samples and ten (83%) and nine (75%) effluent samples at each plant, respectively. Log reduction ratios of Arcobacter 16S rRNA gene in Plant A and Plant B were 1.7 ± 0.9 (n = 10) and 2.3 ± 1.5 (n = 9), respectively. The ciaB gene was detected by quantitative PCR in eleven (92%) and twelve (100%) of 12 influent samples from Plant A and Plant B, respectively, while the pldA gene was detected in eight (67%) and six (50%) influent samples from Plant A and Plant B, respectively. The prevalence of potentially pathogenic bacteria in WWTP effluent indicated the need for disinfection before discharge into the environment.

Original languageEnglish (US)
Article number175
JournalPathogens
Volume8
Issue number4
DOIs
StatePublished - Dec 2019

Fingerprint

Arcobacter
Waste Water
rRNA Genes
Genes
Bacteria
Metagenomics
Bacterial DNA
Polymerase Chain Reaction
Disinfection
Virulence

Keywords

  • Arcobacter
  • Next-generation sequencing
  • Virulence gene
  • Wastewater treatment

ASJC Scopus subject areas

  • Immunology and Allergy
  • Molecular Biology
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Reduction of Arcobacter at two conventional wastewater treatment plants in southern Arizona, USA. / Shrestha, Rajani Ghaju; Sherchan, Samendra P.; Kitajima, Masaaki; Tanaka, Yasuhiro; Gerba, Charles P.; Haramoto, Eiji.

In: Pathogens, Vol. 8, No. 4, 175, 12.2019.

Research output: Contribution to journalArticle

Shrestha, Rajani Ghaju ; Sherchan, Samendra P. ; Kitajima, Masaaki ; Tanaka, Yasuhiro ; Gerba, Charles P. ; Haramoto, Eiji. / Reduction of Arcobacter at two conventional wastewater treatment plants in southern Arizona, USA. In: Pathogens. 2019 ; Vol. 8, No. 4.
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