Reduction of Cryptosporidium, Giardia, and Fecal Indicators by Bardenpho Wastewater Treatment

Bradley William Schmitz, Hitoha Moriyama, Eiji Haramoto, Masaaki Kitajima, Samendra Sherchan, Charles P Gerba, Ian L Pepper

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Increased demand for water reuse and reclamation accentuates the importance for optimal wastewater treatment to limit protozoa in effluents. Two wastewater treatment plants utilizing advanced Bardenpho were investigated over a 12-month period to determine the incidence and reduction of Cryptosporidium, Giardia, Cyclospora, and fecal indicators. Results were compared to conventional facilities that previously operated in the same geographical area. Protozoa (oo)cysts were concentrated using an electronegative filter and subsequently detected by fluorescent microscopy and/or PCR methods. Cryptosporidium and Giardia were frequently detected in raw sewage, but Cyclospora was not detected in any wastewater samples. Facilities with Bardenpho treatment exhibited higher removals of (oo)cysts than facilities utilizing activated sludge or trickling filters. This was likely due to Bardenpho systems having increased solid wasting rates; however, this mechanism cannot be confirmed as sludge samples were not analyzed. Use of dissolved-air-flotation instead of sedimentation tanks did not result in more efficient removal of (oo)cysts. Concentrations of protozoa were compared with each other, Escherichia coli, somatic coliphage, and viruses (pepper mild mottle virus, Aichi virus 1, adenovirus, and polyomaviruses JC and BK). Although significant correlations were rare, somatic coliphage showed the highest potential as an indicator for the abundance protozoa in wastewaters.

Original languageEnglish (US)
JournalEnvironmental Science and Technology
DOIs
StateAccepted/In press - Nov 16 2017

Fingerprint

Protozoa
Wastewater treatment
cyst
Viruses
virus
Wastewater
Settling tanks
wastewater
Reclamation
Sewage
Flotation
Escherichia coli
activated sludge
microscopy
Effluents
Microscopic examination
sewage
sludge
sedimentation
effluent

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Reduction of Cryptosporidium, Giardia, and Fecal Indicators by Bardenpho Wastewater Treatment. / Schmitz, Bradley William; Moriyama, Hitoha; Haramoto, Eiji; Kitajima, Masaaki; Sherchan, Samendra; Gerba, Charles P; Pepper, Ian L.

In: Environmental Science and Technology, 16.11.2017.

Research output: Contribution to journalArticle

Schmitz, Bradley William ; Moriyama, Hitoha ; Haramoto, Eiji ; Kitajima, Masaaki ; Sherchan, Samendra ; Gerba, Charles P ; Pepper, Ian L. / Reduction of Cryptosporidium, Giardia, and Fecal Indicators by Bardenpho Wastewater Treatment. In: Environmental Science and Technology. 2017.
@article{47367faaf67c4b3ca8f241d01e3328ae,
title = "Reduction of Cryptosporidium, Giardia, and Fecal Indicators by Bardenpho Wastewater Treatment",
abstract = "Increased demand for water reuse and reclamation accentuates the importance for optimal wastewater treatment to limit protozoa in effluents. Two wastewater treatment plants utilizing advanced Bardenpho were investigated over a 12-month period to determine the incidence and reduction of Cryptosporidium, Giardia, Cyclospora, and fecal indicators. Results were compared to conventional facilities that previously operated in the same geographical area. Protozoa (oo)cysts were concentrated using an electronegative filter and subsequently detected by fluorescent microscopy and/or PCR methods. Cryptosporidium and Giardia were frequently detected in raw sewage, but Cyclospora was not detected in any wastewater samples. Facilities with Bardenpho treatment exhibited higher removals of (oo)cysts than facilities utilizing activated sludge or trickling filters. This was likely due to Bardenpho systems having increased solid wasting rates; however, this mechanism cannot be confirmed as sludge samples were not analyzed. Use of dissolved-air-flotation instead of sedimentation tanks did not result in more efficient removal of (oo)cysts. Concentrations of protozoa were compared with each other, Escherichia coli, somatic coliphage, and viruses (pepper mild mottle virus, Aichi virus 1, adenovirus, and polyomaviruses JC and BK). Although significant correlations were rare, somatic coliphage showed the highest potential as an indicator for the abundance protozoa in wastewaters.",
author = "Schmitz, {Bradley William} and Hitoha Moriyama and Eiji Haramoto and Masaaki Kitajima and Samendra Sherchan and Gerba, {Charles P} and Pepper, {Ian L}",
year = "2017",
month = "11",
day = "16",
doi = "10.1021/acs.est.7b05876",
language = "English (US)",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",

}

TY - JOUR

T1 - Reduction of Cryptosporidium, Giardia, and Fecal Indicators by Bardenpho Wastewater Treatment

AU - Schmitz, Bradley William

AU - Moriyama, Hitoha

AU - Haramoto, Eiji

AU - Kitajima, Masaaki

AU - Sherchan, Samendra

AU - Gerba, Charles P

AU - Pepper, Ian L

PY - 2017/11/16

Y1 - 2017/11/16

N2 - Increased demand for water reuse and reclamation accentuates the importance for optimal wastewater treatment to limit protozoa in effluents. Two wastewater treatment plants utilizing advanced Bardenpho were investigated over a 12-month period to determine the incidence and reduction of Cryptosporidium, Giardia, Cyclospora, and fecal indicators. Results were compared to conventional facilities that previously operated in the same geographical area. Protozoa (oo)cysts were concentrated using an electronegative filter and subsequently detected by fluorescent microscopy and/or PCR methods. Cryptosporidium and Giardia were frequently detected in raw sewage, but Cyclospora was not detected in any wastewater samples. Facilities with Bardenpho treatment exhibited higher removals of (oo)cysts than facilities utilizing activated sludge or trickling filters. This was likely due to Bardenpho systems having increased solid wasting rates; however, this mechanism cannot be confirmed as sludge samples were not analyzed. Use of dissolved-air-flotation instead of sedimentation tanks did not result in more efficient removal of (oo)cysts. Concentrations of protozoa were compared with each other, Escherichia coli, somatic coliphage, and viruses (pepper mild mottle virus, Aichi virus 1, adenovirus, and polyomaviruses JC and BK). Although significant correlations were rare, somatic coliphage showed the highest potential as an indicator for the abundance protozoa in wastewaters.

AB - Increased demand for water reuse and reclamation accentuates the importance for optimal wastewater treatment to limit protozoa in effluents. Two wastewater treatment plants utilizing advanced Bardenpho were investigated over a 12-month period to determine the incidence and reduction of Cryptosporidium, Giardia, Cyclospora, and fecal indicators. Results were compared to conventional facilities that previously operated in the same geographical area. Protozoa (oo)cysts were concentrated using an electronegative filter and subsequently detected by fluorescent microscopy and/or PCR methods. Cryptosporidium and Giardia were frequently detected in raw sewage, but Cyclospora was not detected in any wastewater samples. Facilities with Bardenpho treatment exhibited higher removals of (oo)cysts than facilities utilizing activated sludge or trickling filters. This was likely due to Bardenpho systems having increased solid wasting rates; however, this mechanism cannot be confirmed as sludge samples were not analyzed. Use of dissolved-air-flotation instead of sedimentation tanks did not result in more efficient removal of (oo)cysts. Concentrations of protozoa were compared with each other, Escherichia coli, somatic coliphage, and viruses (pepper mild mottle virus, Aichi virus 1, adenovirus, and polyomaviruses JC and BK). Although significant correlations were rare, somatic coliphage showed the highest potential as an indicator for the abundance protozoa in wastewaters.

UR - http://www.scopus.com/inward/record.url?scp=85048013506&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048013506&partnerID=8YFLogxK

U2 - 10.1021/acs.est.7b05876

DO - 10.1021/acs.est.7b05876

M3 - Article

C2 - 29847105

AN - SCOPUS:85048013506

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

ER -