Reelin signaling facilitates maturation of CA1 glutamatergic synapses

Shenfeng Qiu, Edwin J. Weeber

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Reelin signaling through the low-density lipoprotein receptor family members, apoliproprotein E receptor 2 (apoER2) and very-low-density lipoprotein receptor (VLDLR), plays a pivotal role in dictating neuronal lamination during embryonic brain development. Recent evidence suggests that this signaling system also plays a role in the postnatal brain to modulate synaptic transmission, plasticity, and cognitive behavior, mostly likely due to a functional coupling with N-methyl-D-aspartate (NMDA) receptors. In this study, we investigated the effects of reelin on the maturation of CA1 glutamatergic function using electrophysiological and biochemical approaches. In cultured hippocampal slices, reelin treatment increased the amplitude of AMPAR-mediated miniature excitatory postsynaptic currents and the evoked AMPA/NMDA receptor current ratios. In addition, reelin treatment also reduced the number of silent synapses, facilitated a developmental switch from NR2B to NR2A of NMDARs, and increased surface expression of AMPARs in CA1 tissue. In cultured hippocampal neurons from reeler embryos, reduced numbers of AMPAR subunit GluR1 and NMDAR subunit NR1 clustering were observed compared with those obtained from wild-type embryos. Supplementing reelin in the reeler culture obliterated these genotypic differences. These results demonstrate that reelin- and lipoprotein receptor-mediated signaling may operate during developmental maturation of hippocampal glutamatergic function and thus represent a potential important mechanism for controlling synaptic strength and plasticity in the postnatal hippocampus.

Original languageEnglish (US)
Pages (from-to)2312-2321
Number of pages10
JournalJournal of Neurophysiology
Volume97
Issue number3
DOIs
StatePublished - Mar 2007
Externally publishedYes

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Neuronal Plasticity
N-Methyl-D-Aspartate Receptors
Synapses
Embryonic Structures
Lipoprotein Receptors
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
LDL Receptors
Excitatory Postsynaptic Potentials
Brain
Synaptic Transmission
Embryonic Development
Cluster Analysis
Hippocampus
Neurons
VLDL receptor
reelin receptor

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Reelin signaling facilitates maturation of CA1 glutamatergic synapses. / Qiu, Shenfeng; Weeber, Edwin J.

In: Journal of Neurophysiology, Vol. 97, No. 3, 03.2007, p. 2312-2321.

Research output: Contribution to journalArticle

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