Regeneration of PAPS for the enzymatic synthesis of sulfated oligosaccharides

Michael D. Burkart, Masayuki Izumi, Eli Chapman, Chun Hung Lin, Chi Huey Wong

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

This paper describes the study of 3'-phosphoadenosine-5'-phosphosulfate (PAPS) regeneration from 3'-phosphoadenosine-5'-phosphate (PAP) for use in practical syntheses of carbohydrate sulfates which are catalyzed by sulfotransferases. Among the regeneration systems, the one with recombinant aryl sulfotransferase proved to be the most practical. This regeneration system was coupled with a sulfotransferase-catalyzed reaction, using a recombinant Nod factor sulfotransferase, for the synthesis of various oligosaccharide sulfates that were further glycosylated using glycosyl-transferases.

Original languageEnglish (US)
Pages (from-to)5565-5574
Number of pages10
JournalJournal of Organic Chemistry
Volume65
Issue number18
DOIs
StatePublished - Sep 8 2000
Externally publishedYes

ASJC Scopus subject areas

  • Organic Chemistry

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