Regional changes in the hippocampal density of AMPA and NMDA receptors across the lifespan of the rat

Gary L. Wenk, Carol A Barnes

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The current study dissected the fascia dentata (FD) and hilar region from the CA and subicular cell fields of the rat and conducted in vitro determinations of the number of binding sites for N-methyl-D-aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4-isoxazole (AMPA) glutamate receptors across the lifespan. We determined the density of binding of [3H]-glutamate or [3H]-AMPA to NMDA or AMPA receptor sites, respectively. The changes reported might be due to either a change in receptor number or an alteration in the binding characteristics of the receptor site with aging. We found an age-related decline in the number of NMDA receptors in the CA1, CA3 and subicular cell regions of the hippocampus, but not in the FD/hilar region, and an age-related decline in the number of AMPA receptors in the FD/hilar region, but not in the CA fields. The decline in the number of NMDA or AMPA receptors that occurs with aging was not a continuous or homogeneous process. These changes in receptor number might underlie selected age-associated changes in sensitivity to drugs that influence hippocampal function as well as to changes in NMDA-dependent long-term potentiation. A thorough understanding of the mechanisms underlying changes in glutamate receptor function in discrete brain regions, using combined neurochemical and electrophysiological methods, may ultimately provide insight into the fundamental substrates of age-associated memory disorders related to hippocampal dysfunction. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalBrain Research
Volume885
Issue number1
DOIs
StatePublished - Dec 1 2000

Fingerprint

alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
AMPA Receptors
N-Methyl-D-Aspartate Receptors
Dentate Gyrus
Glutamate Receptors
N-Methylaspartate
Isoxazoles
Long-Term Potentiation
Memory Disorders
Glutamic Acid
Hippocampus
Binding Sites
Brain
Pharmaceutical Preparations

Keywords

  • Aging
  • AMPA
  • Hippocampus
  • NMDA
  • Receptor
  • Rodents

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regional changes in the hippocampal density of AMPA and NMDA receptors across the lifespan of the rat. / Wenk, Gary L.; Barnes, Carol A.

In: Brain Research, Vol. 885, No. 1, 01.12.2000, p. 1-5.

Research output: Contribution to journalArticle

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