Regional distribution of putative vasopressin receptors in rat brain and pituitary by quantitative autoradiography

R. E. Brinton, K. W. Gee, J. K. Wamsley, T. P. Davis, H. I. Yamamura

Research output: Contribution to journalArticle

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Abstract

Quantitative light microscopic autoradiography was used to map and characterize the distribution of [3H]arginine vasopressin ([3H]AVP) binding sites in the rat brain. HPLC analysis for possible degradation of AVP during binding indicated that addition of specific peptidase inhibitors prevented metabolism of AVP. Binding sites for [3H]AVP were observed in the hypothalamus and pituitary as well as in brain regions where AVP may act as a neuroregulator. Within the hypothalamus, dense AVP binding sites were seen in the suprachiasmatic, supraoptic, and paraventricular nuclei. High specific binding was also apparent in the median eminence tubero-infundibular region and in the posterior lobe of the pituitary. [3H]AVP labeling at possible neuroregulatory sites was observed in the hippocampus, lateral septum, superficial cortex, cerebellum, nucleus tractus solitarious, adenohypophysis, and spinal cord.

Original languageEnglish (US)
Pages (from-to)7248-7252
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number22 I
DOIs
StatePublished - Dec 1 1984
Externally publishedYes

ASJC Scopus subject areas

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