Regulation of apoptosis in the atresia of dominant bovine follicles of the first follicular wave following ovulation

Kelli E. Valdez, S Peder Cuneo, Adele M. Turzillo

Research output: Contribution to journalArticle

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Abstract

During atresia of bovine follicles, granulosa cells are lost through the controlled form of cell death, apoptosis. The purpose of this study was to characterize the regulation of apoptotic death of granulosa cells in dominant bovine follicles during the first wave of follicular development. Dominant follicles were collected from Holstein heifers on days 4, 6 or 8 of the first follicular wave (n = 5/day). Regulation of apoptosis in granulosa cells was examined by annexin V and propidium iodide staining; measurement of relative levels of mRNA encoding Bcl-2, Bcl-xL and Bax; and activity of caspase-3, -8 and -9. Steady-state levels of mRNA encoding four oxidative stress-response proteins were determined. Compared with day 4, the incidence of apoptotic and nonviable granulosa cells tended to increase on day 6, and numbers of nonviable cells were higher on day 8. The ratios of relative levels of mRNA encoding Bcl-2 to Bax and Bcl-xL to Bax were higher on day 6 than days 4 and 8. Activity of caspases-3 and -9 in granulosa cells did not change among the 3 days, while caspase-8 activity decreased on day 8 compared with days 4 and 6. Amounts of GSHPx, MnSOD and Cu/ZnSOD mRNA in granulosa cells were higher on day 8 than day 6. In theca interna, amounts of Cu/ZnSOD mRNA decreased between days 4 and 6. From the decreased production of estradiol and increased numbers of apoptotic and nonviable granulosa cells, we conclude that atresia of the dominant follicle is initiated between days 4 and 6 of the first follicular wave. However, apoptosis of granulosa cells does not appear to be initiated by changes in expression of oxidative stress-response proteins.

Original languageEnglish (US)
Pages (from-to)71-81
Number of pages11
JournalReproduction
Volume130
Issue number1
DOIs
StatePublished - Jul 2005

Fingerprint

Granulosa Cells
Ovulation
Apoptosis
Messenger RNA
Caspase 8
Heat-Shock Proteins
Caspase 3
Oxidative Stress
Theca Cells
Caspase 9
Propidium
Annexin A5
Estradiol
Cell Death
Cell Count
Staining and Labeling
Incidence

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Cell Biology
  • Endocrinology
  • Embryology

Cite this

Regulation of apoptosis in the atresia of dominant bovine follicles of the first follicular wave following ovulation. / Valdez, Kelli E.; Cuneo, S Peder; Turzillo, Adele M.

In: Reproduction, Vol. 130, No. 1, 07.2005, p. 71-81.

Research output: Contribution to journalArticle

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