Regulation of CCK mRNA in the human neuroepithelioma cell line SK-N-MCIXC in response to second messenger activators

Barbara L. Mania-Farnell, Bradley J. Merrill, Pierre N M Konings, Thomas P Davis

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Regulation of cholecystokinin (CCK) expression was studied in the human neuroepithelioma cell line SK-N-MCIXC. The cells were treated with the phosphodiesterase inhibitor isobutyl-methylxanthine and the tumor promoting phorbol ester, phorbol-12-myristate 13-acetate; activators of the cyclic AMP (cAMP) and protein kinase C (PKC) second messenger pathways, respectively. Levels of CCK mRNA were determined after 6, 12 and 24 hour drug treatments, with Northern blot analysis using human CCK cDNA hybridization probes. Activation of both cAMP and PKC second messenger pathways increased CCK mRNA levels in SK-N-MCIXC cells. These results indicate that the levels of CCK mRNA in SK-N-MCIXC cells are regulated by cAMP and PKC dependent mechanisms.

Original languageEnglish (US)
Pages (from-to)65-68
Number of pages4
JournalFEBS Letters
Volume335
Issue number1
DOIs
StatePublished - Nov 29 1993

Fingerprint

Cholecystokinin
Second Messenger Systems
Adenylate Kinase
Cells
Cell Line
Messenger RNA
Cyclic AMP
Protein Kinase C
Drug therapy
Phosphodiesterase Inhibitors
Phorbol Esters
Northern Blotting
Tumors
Acetates
Complementary DNA
Chemical activation
Pharmaceutical Preparations
Neoplasms

Keywords

  • Cholecystokinin
  • Gene expression
  • Neuronal cell line
  • Neuropeptide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Regulation of CCK mRNA in the human neuroepithelioma cell line SK-N-MCIXC in response to second messenger activators. / Mania-Farnell, Barbara L.; Merrill, Bradley J.; Konings, Pierre N M; Davis, Thomas P.

In: FEBS Letters, Vol. 335, No. 1, 29.11.1993, p. 65-68.

Research output: Contribution to journalArticle

Mania-Farnell, Barbara L. ; Merrill, Bradley J. ; Konings, Pierre N M ; Davis, Thomas P. / Regulation of CCK mRNA in the human neuroepithelioma cell line SK-N-MCIXC in response to second messenger activators. In: FEBS Letters. 1993 ; Vol. 335, No. 1. pp. 65-68.
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