Regulation of dual-specificity phosphatases M3/6 and hVH5 by phorbol esters. Analysis of a delta-like domain

Thomas R. Johnson, Joseph R. Biggs, Sarah E. Winbourn, Andrew Kraft

Research output: Contribution to journalArticle

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Abstract

Treatment of leukemic cells with phorbol 12-myristate 13-acetate (PMA) induces a short-lived phosphorylation and activation of stress-activated protein kinase (SAPK) and cellular differentiation. To investigate whether the rapid deactivation of SAPK results from dephosphorylation by dual-specificity phosphatases (DSPs), we studied regulation of the DSP hVH5 and its murine orthologue M3/6 in K562 human leukemia cells. PMA treatment rapidly induced hVH5 transcripts in these cells, and induced expression of M3/6 completely inhibited PMA-stimulated phosphorylation of SAPK, suggesting a feedback loop to control SAPK activity. Using both stable cell lines and transient transfection we demonstrate that activation of SAPK rapidly stimulated phosphorylation of M3/6. This phosphorylatlon did not regulate the half-life of total cellular M3/6, hVH5 and M3/6 shares with all sequenced mammalian DSPs an amino acid motif, XILPXLXL, located approximately 80 amino acids from the active site. The hVH5-M3/6 sequence, RILPHLYL, shares significant homology with the SAPK binding site of the c-Jun protein, called the delta domain. This motif was found to be important for DSP function, because deletion of RILPHLYL inhibits SAPK-mediated phosphorylation of M3/6, and deletion of this sequence or mutation of the LYL portion blocks the ability of this phosphatase to dephosphorylate SAPK.

Original languageEnglish (US)
Pages (from-to)31755-31762
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number41
StatePublished - Oct 13 2000
Externally publishedYes

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Dual Specificity Phosphatase 6
Dual-Specificity Phosphatases
Phorbol Esters
Heat-Shock Proteins
Protein Kinases
Phosphorylation
Acetates
Chemical activation
Cells
Proto-Oncogene Proteins c-jun
Amino Acids
Amino Acid Motifs
Sequence Deletion
Phosphoric Monoester Hydrolases
Protein Binding
Transfection
Half-Life
Catalytic Domain
Leukemia
Binding Sites

ASJC Scopus subject areas

  • Biochemistry

Cite this

Regulation of dual-specificity phosphatases M3/6 and hVH5 by phorbol esters. Analysis of a delta-like domain. / Johnson, Thomas R.; Biggs, Joseph R.; Winbourn, Sarah E.; Kraft, Andrew.

In: Journal of Biological Chemistry, Vol. 275, No. 41, 13.10.2000, p. 31755-31762.

Research output: Contribution to journalArticle

Johnson, Thomas R. ; Biggs, Joseph R. ; Winbourn, Sarah E. ; Kraft, Andrew. / Regulation of dual-specificity phosphatases M3/6 and hVH5 by phorbol esters. Analysis of a delta-like domain. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 41. pp. 31755-31762.
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