Regulation of glycolytic metabolism during long-term primary culture of renal proximal tubule cells

M. D. Aleo, R. G. Schnellmann

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Adequate oxygenation was a major factor regulating the induction of glycolytic metabolism in primary cultures of rabbit renal proximal tubule cells during short-term (<1 day) and long-term (1-7 day) culture. As measured by cellular lactate content, glucose consumption, and lactate dehydrogenase activity, less glycolytic metabolism was induced in cultured cells that were constantly aerated than in cells that were held stationary. When oxidative metabolism is supported by providing 2-10 mM hepatanoate (HEP) as a metabolic substrate glycolytic metabolism further decreased during short-term, but not long-term culture. Cellular proliferation did not play a major role in regulating the induction of glycolytic metabolism, since glycolytic metabolism increased before cell growth had occurred, did not decline once logarithmic cell growth had ceased, and was stimulated less by cell growth than by inadequate oxygenation. Fructose-1,6-bis- phosphatase and alkaline phosphatase, representative markers of gluconeogenic and brush-border membrane enzyme activities, respectively, declined during culture regardless of culture conditions or the presence of HEP. Therefore, glycolytic metabolism can be effectively minimized by constantly aerating cultured proximal tubule cells and can be further reduced by the addition of HEP during short-term culture.

Original languageEnglish (US)
Pages (from-to)F77-F85
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume262
Issue number1 31-1
DOIs
StatePublished - 1992
Externally publishedYes

Keywords

  • Fatty acid
  • Glucose
  • Hypoxia
  • Lactate
  • Oxidative metabolism
  • Oxygen

ASJC Scopus subject areas

  • Physiology

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