TY - JOUR
T1 - Regulation of hemolymph osmolality in feral and domestic honeybees, Apis mellifera L. (Hymenoptera
T2 - Apidae)
AU - Atmowidjojo, Anita H.
AU - Erickson, Eric H.
AU - Wheeler, Diana E.
AU - Cohen, Allen C.
N1 - Funding Information:
We are grateful to the Government of Indonesia and USAID for supporting grant for training for A.H. Atmowidjojo, the USDA Carl Hayden Bee Research Center, Tucson, AZ and the USDA Cotton Insect Pest Mgt/Biological Control, Phoenix, AZ, USA for allowing this research. We also thank Dr Nancy Moran for her helpful advice, and Harold Don, John Edwards, Joe Martin and Clark Newman for their technical assistance.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1999/2
Y1 - 1999/2
N2 - Experiments were conducted to compare the ability of unmanaged fetal and managed domestic honeybees in the arid Southwest to regulate hemolymph solutes, osmolality, and uric acid. Honeybees from feral and domestic colonies were desiccated (at 30°C and 0% humidity) for 2 h with undesiccated bees held as controls. Hemolymph osmolality, proteins, amino acid, and uric acid concentrations were analyzed. Hemolymph osmotic pressures of desiccated feral bees were significantly lower than those of domestic honeybees (P < 0.004). There was a significant difference in hemolymph protein concentrations between undesiccated and desiccated honeybees (P < 0.001). The hemolymph concentration of amino acids was significantly higher in undesiccated bees than in desiccated honeybees (P < 0.0031). There were no differences in uric acid concentrations between feral and domestic bees, and between desiccated and undesiccated honeybees. Hence, differences in temperature tolerance and water balance between feral and domestic honey bees are not explained by differential regulation of hemolymph osmolality, proteins, or free amino acids, or by regulation of uric acid excretion.
AB - Experiments were conducted to compare the ability of unmanaged fetal and managed domestic honeybees in the arid Southwest to regulate hemolymph solutes, osmolality, and uric acid. Honeybees from feral and domestic colonies were desiccated (at 30°C and 0% humidity) for 2 h with undesiccated bees held as controls. Hemolymph osmolality, proteins, amino acid, and uric acid concentrations were analyzed. Hemolymph osmotic pressures of desiccated feral bees were significantly lower than those of domestic honeybees (P < 0.004). There was a significant difference in hemolymph protein concentrations between undesiccated and desiccated honeybees (P < 0.001). The hemolymph concentration of amino acids was significantly higher in undesiccated bees than in desiccated honeybees (P < 0.0031). There were no differences in uric acid concentrations between feral and domestic bees, and between desiccated and undesiccated honeybees. Hence, differences in temperature tolerance and water balance between feral and domestic honey bees are not explained by differential regulation of hemolymph osmolality, proteins, or free amino acids, or by regulation of uric acid excretion.
KW - Apis mellifera
KW - Feral honeybees
KW - Hemolymph amino acids
KW - Hemolymph proteins
KW - Osmolality
KW - Temperature tolerance
KW - Uric acid
KW - Water balance
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U2 - 10.1016/S1095-6433(98)10179-4
DO - 10.1016/S1095-6433(98)10179-4
M3 - Article
AN - SCOPUS:0032929490
VL - 122
SP - 227
EP - 233
JO - Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
JF - Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
SN - 1095-6433
IS - 2
ER -