Regulation of human epsilon germline transcription

Role of b-cell-specific activator protein

Lucia De Monte, Cortlandt P. Thienes, Silvia Monticelli, Meinrad Busslinger, Hannah J. Gould, Donata Vercelli

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Germline transcripts initiate from promoters upstream of the immunoglobulin switch region, and are necessary to target the appropriate switch region for recombination and switching. Different cytokines activate transcription at the appropriate germline promoter. Because binding sites for B-cell-specific activator protein (BSAP) are located upstream of several switch regions in the immunoglobulin heavy chain gene cluster, BSAP might play a role in the regulation of germline transcription and isotype switching. We investigated whether BSAP plays a role in the transcriptional regulation of the ε germline promoter in human B cells. Our results showed that BSAP plays a role in both IL-4-dependent induction and CD40-mediated upregulation of human ε germline transcription. BSAP is unique among the transcription factors that regulate ε germline expression, because it is B cell specific, and is at the merging point of two signalling pathways that are critical for IgE switching.

Original languageEnglish (US)
Pages (from-to)35-38
Number of pages4
JournalInternational Archives of Allergy and Immunology
Volume113
Issue number1-3
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Fingerprint

PAX5 Transcription Factor
Proteins
Immunoglobulin Switch Region
B-Lymphocytes
Immunoglobulin Class Switching
Immunoglobulin Heavy Chain Genes
Critical Pathways
Multigene Family
Interleukin-4
Immunoglobulin E
Genetic Recombination
Transcription Factors
Up-Regulation
Binding Sites
Cytokines

Keywords

  • B lymphocytes
  • Cytokines
  • Gene regulation
  • IgE
  • Transcription factors

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Regulation of human epsilon germline transcription : Role of b-cell-specific activator protein. / De Monte, Lucia; Thienes, Cortlandt P.; Monticelli, Silvia; Busslinger, Meinrad; Gould, Hannah J.; Vercelli, Donata.

In: International Archives of Allergy and Immunology, Vol. 113, No. 1-3, 01.01.1997, p. 35-38.

Research output: Contribution to journalArticle

De Monte, Lucia ; Thienes, Cortlandt P. ; Monticelli, Silvia ; Busslinger, Meinrad ; Gould, Hannah J. ; Vercelli, Donata. / Regulation of human epsilon germline transcription : Role of b-cell-specific activator protein. In: International Archives of Allergy and Immunology. 1997 ; Vol. 113, No. 1-3. pp. 35-38.
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