Regulation of na+, k+-atpase gene expression by insulin in vascular smooth muscle cells

Prakash R. Tirupattur, Jeffrey L. Ram, Paul R. Standley, James R. Sowers

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Na+, K+-ATPase gene expression and activity and its modulation by insulin were investigated in vascular smooth muscle cells (VSMC). Messenger RNAs encoding for αand α2-isoforms of Na+, K+- ATPase were identified in a pure population of cultured VSMC (a7r5 cells). The predominant isoform in VSMC was alf but only α2 was regulated by insulin. Insulin treatment of VSMC for 1 and 6 h caused significant increases in mRNA encoding the α2-subunit of Na+, K+-ATPase (n = 7, P <.01). Under similar conditions, insulin had no effect on a:1-Na+, K+-ATPase mRNA levels. VSMC treated with insulin exhibited a significant stimulation of ouabain inhibitable ATPase activity (1 h insulin, n = 3, v control, n = 3, P <.025; 6 h insulin, n = 3, v control, n = 3, P <.05). Changes in insulin regulation of Na+, K+-ATPase gene expression in vascular smooth muscle may be an important factor in the development of hypertension in diabetes. Am J Hypertens.

Original languageEnglish (US)
Pages (from-to)626-629
Number of pages4
JournalAmerican Journal of Hypertension
Volume6
Issue number7
DOIs
StatePublished - Sep 1993

Keywords

  • Insulin
  • K-atpase
  • Mrna
  • Na
  • Ouabain
  • Polymerase chain reaction
  • Southern blot
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Internal Medicine

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