Regulation of Na+,K+-ATPase gene expression by insulin in vascular smooth muscle cells

Prakash R. Tirupattur, Jeffrey L. Ram, Paul R Standley, James R. Sowers

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Na+,K+-ATPase gene expression and activity and its modulation by insulin were investigated in vascular smooth muscle cells (VSMC). Messenger RNAs encoding for α1- and α2-isoforms of Na+,K+-ATPase were identified in a pure population of cultured VSMC (a7r5 cells). The predominant isoform in VSMC was α1 but only α2 was regulated by insulin. Insulin treatment of VSMC for 1 and 6 h caused significant increases in mRNA encoding the α1-subunit of Na+,K+-ATPase (n = 7, P < .01). Under similar conditions, insulin had no effect on α1-Na+,K+-ATPase mRNA levels. VSMC treated with insulin exhibited a significant stimulation of ouabain inhibitable ATPase activity (1 h insulin, n = 3, v control, n = 3, P < .025; 6 h insulin, n = 3, v control, n = 3, P < .05). Changes in insulin regulation of Na+,K+-ATPase gene expression in vascular smooth muscle may be an important factor in the development of hypertension in diabetes.

Original languageEnglish (US)
Pages (from-to)626-629
Number of pages4
JournalAmerican Journal of Hypertension
Volume6
Issue number7 PART 1
StatePublished - Jul 1993
Externally publishedYes

Fingerprint

Vascular Smooth Muscle
Smooth Muscle Myocytes
Insulin
Gene Expression
Messenger RNA
Protein Isoforms
sodium-translocating ATPase
Ouabain
Adenosine Triphosphatases
Hypertension
Population

Keywords

  • insulin
  • K-ATPase
  • mRNA
  • Na
  • ouabain
  • polymerase chain reaction
  • Southern blot
  • vascular smooth muscle

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Regulation of Na+,K+-ATPase gene expression by insulin in vascular smooth muscle cells. / Tirupattur, Prakash R.; Ram, Jeffrey L.; Standley, Paul R; Sowers, James R.

In: American Journal of Hypertension, Vol. 6, No. 7 PART 1, 07.1993, p. 626-629.

Research output: Contribution to journalArticle

Tirupattur, Prakash R. ; Ram, Jeffrey L. ; Standley, Paul R ; Sowers, James R. / Regulation of Na+,K+-ATPase gene expression by insulin in vascular smooth muscle cells. In: American Journal of Hypertension. 1993 ; Vol. 6, No. 7 PART 1. pp. 626-629.
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