Regulation of skeletal α-actin promoter in young chickens during hypertrophy caused by stretch overload

J. A. Carson, Z. Yan, F. W. Booth, M. E. Coleman, R. J. Schwartz, Craig S Stump

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Anterior latissimus dorsi (ALD) muscles of 3-wk-old male chickens were injected with plasmids containing various lengths of the chicken skeletal α- actin promoter (ranging from -2,090 to -77 relative to the transcription start site) driving luciferase. Hypertrophy of the left ALD muscle was induced by attaching a weight (11% of body wt) to the left wing of each chicken, with the unweighted contralateral wing serving the control. Six days of stretch overload significantly increased muscle mass 110%. Luciferase activity from the -2,090 actin-luciferase chimeric gene increased 127% compared with the contralateral control ALD muscle. Luciferase activities driven by the -424, -202, and -99 actin promoters were 179, 134, and 378% higher, respectively, in the stretched ALD muscle than in the contralateral control ALD muscle. Luciferase activity from the -77 deletion construct was not different between stretched and control muscles. These data indicate that the gene region responding to stretch is downstream of -99 and imply, but do not conclusively prove, that the region between -99 and -77, which contains serum response element 1, contributes to the stretch-induced increase in skeletal α-actin promoter activity in the ALD muscle.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume268
Issue number4 37-4
StatePublished - 1995
Externally publishedYes

Fingerprint

Hypertrophy
Superficial Back Muscles
Muscle
Actins
Chickens
Muscles
Luciferases
Genes
Serum Response Element
Transcription Initiation Site
Plasmids
Body Weight

Keywords

  • contractile protein
  • exercise
  • gene
  • muscle

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Regulation of skeletal α-actin promoter in young chickens during hypertrophy caused by stretch overload. / Carson, J. A.; Yan, Z.; Booth, F. W.; Coleman, M. E.; Schwartz, R. J.; Stump, Craig S.

In: American Journal of Physiology - Cell Physiology, Vol. 268, No. 4 37-4, 1995.

Research output: Contribution to journalArticle

Carson, J. A. ; Yan, Z. ; Booth, F. W. ; Coleman, M. E. ; Schwartz, R. J. ; Stump, Craig S. / Regulation of skeletal α-actin promoter in young chickens during hypertrophy caused by stretch overload. In: American Journal of Physiology - Cell Physiology. 1995 ; Vol. 268, No. 4 37-4.
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