Regulation of sphingosine 1-phosphate-induced endothelial cytoskeletal rearrangement and barrier enhancement by S1P1 receptor, PI3 kinase, Tiam1/Rac1, and α-actinin

Patrick A. Singleton, Steven M. Dudek, Eddie T. Chiang, Joe GN Garcia

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

Endothelial cell (EC) barrier dysfunction results in increased vascular permeability observed in inflammation, tumor angiogenesis, and atherosclerosis. The platelet-derived phospholipid sphingosine-1-phosphate (S1P) decreases EC permeability in vitro and in vivo and thus has obvious therapeutic potential. We examined S1P-mediated human pulmonary artery EC signaling and barrier regulation in caveolin-enriched microdomains (CEM). Immunoblotting from S1P-treated EC revealed S1P-mediated rapid recruitment (1 μM, 5 min) to CEMs of the S1P receptors S1P1 and S1P3, p110 PI3 kinase α and β catalytic subunits, the Rac1 GEF, Tiam1, and α-actinin isoforms 1 and 4. Immunoprecipitated p110 PI3 kinase catalytic subunits from S1P-treated EC exhibited PIP3 production in CEMs. Immunoprecipitation of S1P receptors from CEM fractions revealed complexes containing Tiam1 and S1P1. PI3 kinase inhibition (LY294002) attenuated S1P-induced Tiam1 association with S1P1, Tiam1/Rac1 activation, α-actinin-1/4 recruitment, and EC barrier enhancement. Silencing of either S1P1 or Tiam1 expression resulted in the loss of S1P-mediated Rac1 activation and α-actinin-1/4 recruitment to CEM. Finally, silencing S1P1, Tiam1, or both α-actinin isoforms 1/4 inhibits S1P-induced cortical F-actin rearrangement and S1P-mediated barrier enhancement. Taken together, these results suggest that S1P-induced recruitment of S1P1 to CEM fractions promotes PI3 kinase-mediated Tiam1/Rac1 activation required for α-actinin-1/4-regulated cortical actin rearrangement and EC barrier enhancement.

Original languageEnglish (US)
Pages (from-to)1646-1656
Number of pages11
JournalFASEB Journal
Volume19
Issue number12
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Lysosphingolipid Receptors
Actinin
sphingosine
Phosphatidylinositol 3-Kinases
phosphotransferases (kinases)
Endothelial cells
phosphates
receptors
Caveolins
Endothelial Cells
endothelial cells
Chemical activation
protein subunits
Actins
Catalytic Domain
Protein Isoforms
actin
sphingosine 1-phosphate
permeability
Cell signaling

Keywords

  • α-actinin
  • Cytoskeleton
  • PI3 kinase
  • S1P
  • S1P/Edg1 receptor
  • Tiam1

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Regulation of sphingosine 1-phosphate-induced endothelial cytoskeletal rearrangement and barrier enhancement by S1P1 receptor, PI3 kinase, Tiam1/Rac1, and α-actinin. / Singleton, Patrick A.; Dudek, Steven M.; Chiang, Eddie T.; Garcia, Joe GN.

In: FASEB Journal, Vol. 19, No. 12, 10.2005, p. 1646-1656.

Research output: Contribution to journalArticle

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abstract = "Endothelial cell (EC) barrier dysfunction results in increased vascular permeability observed in inflammation, tumor angiogenesis, and atherosclerosis. The platelet-derived phospholipid sphingosine-1-phosphate (S1P) decreases EC permeability in vitro and in vivo and thus has obvious therapeutic potential. We examined S1P-mediated human pulmonary artery EC signaling and barrier regulation in caveolin-enriched microdomains (CEM). Immunoblotting from S1P-treated EC revealed S1P-mediated rapid recruitment (1 μM, 5 min) to CEMs of the S1P receptors S1P1 and S1P3, p110 PI3 kinase α and β catalytic subunits, the Rac1 GEF, Tiam1, and α-actinin isoforms 1 and 4. Immunoprecipitated p110 PI3 kinase catalytic subunits from S1P-treated EC exhibited PIP3 production in CEMs. Immunoprecipitation of S1P receptors from CEM fractions revealed complexes containing Tiam1 and S1P1. PI3 kinase inhibition (LY294002) attenuated S1P-induced Tiam1 association with S1P1, Tiam1/Rac1 activation, α-actinin-1/4 recruitment, and EC barrier enhancement. Silencing of either S1P1 or Tiam1 expression resulted in the loss of S1P-mediated Rac1 activation and α-actinin-1/4 recruitment to CEM. Finally, silencing S1P1, Tiam1, or both α-actinin isoforms 1/4 inhibits S1P-induced cortical F-actin rearrangement and S1P-mediated barrier enhancement. Taken together, these results suggest that S1P-induced recruitment of S1P1 to CEM fractions promotes PI3 kinase-mediated Tiam1/Rac1 activation required for α-actinin-1/4-regulated cortical actin rearrangement and EC barrier enhancement.",
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AU - Singleton, Patrick A.

AU - Dudek, Steven M.

AU - Chiang, Eddie T.

AU - Garcia, Joe GN

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N2 - Endothelial cell (EC) barrier dysfunction results in increased vascular permeability observed in inflammation, tumor angiogenesis, and atherosclerosis. The platelet-derived phospholipid sphingosine-1-phosphate (S1P) decreases EC permeability in vitro and in vivo and thus has obvious therapeutic potential. We examined S1P-mediated human pulmonary artery EC signaling and barrier regulation in caveolin-enriched microdomains (CEM). Immunoblotting from S1P-treated EC revealed S1P-mediated rapid recruitment (1 μM, 5 min) to CEMs of the S1P receptors S1P1 and S1P3, p110 PI3 kinase α and β catalytic subunits, the Rac1 GEF, Tiam1, and α-actinin isoforms 1 and 4. Immunoprecipitated p110 PI3 kinase catalytic subunits from S1P-treated EC exhibited PIP3 production in CEMs. Immunoprecipitation of S1P receptors from CEM fractions revealed complexes containing Tiam1 and S1P1. PI3 kinase inhibition (LY294002) attenuated S1P-induced Tiam1 association with S1P1, Tiam1/Rac1 activation, α-actinin-1/4 recruitment, and EC barrier enhancement. Silencing of either S1P1 or Tiam1 expression resulted in the loss of S1P-mediated Rac1 activation and α-actinin-1/4 recruitment to CEM. Finally, silencing S1P1, Tiam1, or both α-actinin isoforms 1/4 inhibits S1P-induced cortical F-actin rearrangement and S1P-mediated barrier enhancement. Taken together, these results suggest that S1P-induced recruitment of S1P1 to CEM fractions promotes PI3 kinase-mediated Tiam1/Rac1 activation required for α-actinin-1/4-regulated cortical actin rearrangement and EC barrier enhancement.

AB - Endothelial cell (EC) barrier dysfunction results in increased vascular permeability observed in inflammation, tumor angiogenesis, and atherosclerosis. The platelet-derived phospholipid sphingosine-1-phosphate (S1P) decreases EC permeability in vitro and in vivo and thus has obvious therapeutic potential. We examined S1P-mediated human pulmonary artery EC signaling and barrier regulation in caveolin-enriched microdomains (CEM). Immunoblotting from S1P-treated EC revealed S1P-mediated rapid recruitment (1 μM, 5 min) to CEMs of the S1P receptors S1P1 and S1P3, p110 PI3 kinase α and β catalytic subunits, the Rac1 GEF, Tiam1, and α-actinin isoforms 1 and 4. Immunoprecipitated p110 PI3 kinase catalytic subunits from S1P-treated EC exhibited PIP3 production in CEMs. Immunoprecipitation of S1P receptors from CEM fractions revealed complexes containing Tiam1 and S1P1. PI3 kinase inhibition (LY294002) attenuated S1P-induced Tiam1 association with S1P1, Tiam1/Rac1 activation, α-actinin-1/4 recruitment, and EC barrier enhancement. Silencing of either S1P1 or Tiam1 expression resulted in the loss of S1P-mediated Rac1 activation and α-actinin-1/4 recruitment to CEM. Finally, silencing S1P1, Tiam1, or both α-actinin isoforms 1/4 inhibits S1P-induced cortical F-actin rearrangement and S1P-mediated barrier enhancement. Taken together, these results suggest that S1P-induced recruitment of S1P1 to CEM fractions promotes PI3 kinase-mediated Tiam1/Rac1 activation required for α-actinin-1/4-regulated cortical actin rearrangement and EC barrier enhancement.

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KW - S1P/Edg1 receptor

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