Regulation of the Dictyostelium glycogen phosphorylase 2 gene by cyclic AMP

J. F. Sucic, Ornella Selmin, C. L. Rutherford

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A crucial developmental event in the cellular slime mold, Dictyostelium discoideum, is glycogen degradation. The enzyme that catalyzes this degradation, glycogen phosphorylase 2 (gp-2), is developmentally regulated and cAMP appears to be involved in this regulation. We have examined several aspects of the cAMP regulation of gp-2. We show that addition of exogenous cAMP to aggregation competent amoebae induced the appearance of gp-2 mRNA. The induction of gp-2 mRNA occurred within 1 and 1.5 h after the initial exposure to cAMP. Exposure to exogenous cAMP concentrations as low as 1.0 μM could induce gp-2 mRNA. We also examined the molecular mechanism through which cAMP induction of gp-2 occurs. Induction of gp-2 appears to result from a mechanism that does not require intracellular cAMP signaling, and may occur directly through a cAMP binding protein without the requirement of any intracellular signalling. We also examined the promoter region of the gp-2 gene for cis-acting elements that are involved in the cAMP regulation of gp- 2. A series of deletions of the promoter were fused to a luciferase reporter gene and then analyzed for cAMP responsiveness. The results indicated that a region from -258 nucleotides to the transcriptional start site is sufficient for essentially full activity and appears to carry all necessary cis-acting sites for cAMP induction. Further deletion of 58 nucleotides from the 5' end, results in fivefold less activity in the presence of cAMP. Deletion of the next 104 nucleotides eliminates the cAMP response entirely.

Original languageEnglish (US)
Pages (from-to)313-322
Number of pages10
JournalDevelopmental Genetics
Volume14
Issue number4
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Glycogen Phosphorylase
Dictyostelium
Cyclic AMP
Genes
Nucleotides
Messenger RNA
Dictyosteliida
Amoeba
Luciferases
Glycogen
Reporter Genes
Genetic Promoter Regions
Carrier Proteins
Enzymes

Keywords

  • Dictyostelium
  • gene regulation
  • glycogen phosphorylase

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics

Cite this

Regulation of the Dictyostelium glycogen phosphorylase 2 gene by cyclic AMP. / Sucic, J. F.; Selmin, Ornella; Rutherford, C. L.

In: Developmental Genetics, Vol. 14, No. 4, 1993, p. 313-322.

Research output: Contribution to journalArticle

Sucic, J. F. ; Selmin, Ornella ; Rutherford, C. L. / Regulation of the Dictyostelium glycogen phosphorylase 2 gene by cyclic AMP. In: Developmental Genetics. 1993 ; Vol. 14, No. 4. pp. 313-322.
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