The molecular and genetic basis of large differences in the concentration of P lysozyme in the small intestine has been investigated by crossing inbred strains of two species of house mouse (genus Mus). The concentration of P in domesticus is about 130-fold higher than in castaneus. An autosomal genetic element determining the concentration of P has been identified and named the P lysozyme regulator, Lzp-r. The level of P in interspecific hybrids (domesticus X castaneus) as well as in certain classes of backcross progeny is intermediate relative to parental levels, which shows that the two alleles of Lzp-rare inherited additively. There are two forms of P lysozyme in the intestine of the interspecific hybrid - one having the heat stability of domesticus P, the other being more stable and presumably the product of the castaneus P locus. These two forms occur in equal amounts, and it appears that Lzp-racts in trans. The linkage of Lzp-rto three structural genes (Lzp-s,Lzm-s1, and Lzm-s2), one specifying P lysozyme and two specifying M lysozymes, was shown by electrophoretic analysis of backcrosses involving domesticus and castaneus and also domesticus and spretus. The role of regulatory mutations in evolution is discussed in light of these results.
|Original language||English (US)|
|Number of pages||13|
|State||Published - Jan 1 1987|
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