Relation of in vitro colony survival to clinical response in a prospective trial of single-agent chemotherapy for metastatic melanoma

F. L. Meyskens, L. Loescher, T. E. Moon, B. Takasugi, S. E. Salmon

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

We have used the effect of therapeutic agents on clonogenic growth in agar to discriminate between active and inactive agents for malignant melanoma. We report a prospective study of single-agent chemotherapy for metastatic melanoma. Forty-five separate in vitro/in vivo correlative trials were conducted in 34 patients. A number of agents were used in these evaluations, including actinomycin D, Amsacrine, bisantrene, mitoxantrone, BCNU, vinblastine, vindesine, 5-fluorouracil, MGBG, etoposide, interferon, tamoxifen, and 13-cis-retinoic acid. At the 'cut-off' concentration, a colony survival <30% was designated as 'sensitivity' and >30% as 'resistance'. Clinical sensitivity was designated to include complete, partial, and mixed responses and was predicted in eight of 18 trials (44%). Clinical resistance (nonresponse) was predicted correctly in 24 of 27 cases (89%). Using Fisher's exact test the association of in vitro and in vivo results was significant (P=.05). These results offer further support for the concept that clonogenic assays may help select useful agents for clinical trials in metastatic melanoma.

Original languageEnglish (US)
Pages (from-to)1223-1228
Number of pages6
JournalJournal of Clinical Oncology
Volume2
Issue number11
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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