Relation of in vitro colony survival to clinical response in a prospective trial of single-agent chemotherapy for metastatic melanoma

F. L. Meyskens, L. Loescher, T. E. Moon, B. Takasugi, S. E. Salmon

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We have used the effect of therapeutic agents on clonogenic growth in agar to discriminate between active and inactive agents for malignant melanoma. We report a prospective study of single-agent chemotherapy for metastatic melanoma. Forty-five separate in vitro/in vivo correlative trials were conducted in 34 patients. A number of agents were used in these evaluations, including actinomycin D, Amsacrine, bisantrene, mitoxantrone, BCNU, vinblastine, vindesine, 5-fluorouracil, MGBG, etoposide, interferon, tamoxifen, and 13-cis-retinoic acid. At the 'cut-off' concentration, a colony survival <30% was designated as 'sensitivity' and >30% as 'resistance'. Clinical sensitivity was designated to include complete, partial, and mixed responses and was predicted in eight of 18 trials (44%). Clinical resistance (nonresponse) was predicted correctly in 24 of 27 cases (89%). Using Fisher's exact test the association of in vitro and in vivo results was significant (P=.05). These results offer further support for the concept that clonogenic assays may help select useful agents for clinical trials in metastatic melanoma.

Original languageEnglish (US)
Pages (from-to)1223-1228
Number of pages6
JournalJournal of Clinical Oncology
Volume2
Issue number11
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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