Relative abundance of bovine Hsp70 mRNA and protein

Jesus A. Gutierrez, Vincent Guerriero

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A solution hybridization assay to measure bovine Hsp70 mRNA levels is used to demonstrate that skeletal muscle contains the highest amount of this mRNA while brain has the lowest amount and the level of Hsp70 mRNA is heat inducible in bovine skeletal muscle. Furthermore, this assay allowed for the comparison of relative Hsp70 protein and mRNA levels. Relative transcript levels compared to relative Hsp70 protein levels in tissues demonstrated 50- to 200-fold differences. These results, then, approximate the magnitude of the previously reported preferential translation of Hsp70 mRNA.

Original languageEnglish (US)
Pages (from-to)239-242
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1260
Issue number2
DOIs
StatePublished - Jan 25 1995

Fingerprint

Messenger RNA
Skeletal Muscle
Proteins
Muscle
Assays
Protein Biosynthesis
Hot Temperature
Brain
Tissue

Keywords

  • (Bovine)
  • Hsp70
  • mRNA level
  • Protein level

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Relative abundance of bovine Hsp70 mRNA and protein. / Gutierrez, Jesus A.; Guerriero, Vincent.

In: BBA - Gene Structure and Expression, Vol. 1260, No. 2, 25.01.1995, p. 239-242.

Research output: Contribution to journalArticle

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